One hundred and forty-nine strains of nocardiae, freshly isolated from soil samples obtained from a number of countries with either tropical or temperate climates, and from rubber pipe seals, were compared with appropriate marker cultures in a numerical phenetic study using 156 unit characters. Marker strains were chosen to represent the complex, other species and related taxa in an effort both to classify the new soil isolates and, possibly, clarify the structure of the heterogeneous N. complex. The data were examined using the simple matching (Ssm) and pattern (D) coefficients, and clustering was achieved using both single and average linkage algorithms. Cluster composition was not markedly affected by either of the coefficients or clustering methods. The estimated test error of 7.1 % was rather high and could account for a few apparently anomalous results. The 16 defined clusters, containing 185 of the 197 strains studied, were divided into seven major and nine minor clusters, four of which were further subdivided into two subclusters. Marker strains allowed four clusters to be designated as , seven as species and one each as and species. Twelve strains formed single member clusters including the type strains of and . The majority of the soil and rubber isolates were recovered in the major clusters labelled and species and clusters of soil isolates without marker strains seem to represent new centres of variation. The study highlights the need for additional repoducible tests to help both define and determine the status of defined clusters within the complex which would considerably benefit both the ecological and epidemiological study of these organisms.


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