1887

Abstract

A simple chromatographic procedure has been devised to separate γ-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase, allowing the measurement of the former in crude extracts. Analysis of a number of strains of has demonstrated that gene pro codes for γ-glutamyl phosphate reductase and for γ-glutamyl kinase. Introduction of a ColEl hybrid plasmid containing the region into a strain with a chromosomal deletion of led to 3- and 17-fold increases in the specific activities of γ-glutamyl kinase and γ-glutamyl phosphate reductase, respectively.

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/content/journal/micro/10.1099/00221287-118-2-287
1980-06-01
2020-11-25
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-2-287
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