1887

Abstract

A simple chromatographic procedure has been devised to separate -glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase, allowing the measurement of the former in crude extracts. Analysis of a number of strains of has demonstrated that gene pro codes for -glutamyl phosphate reductase and for -glutamyl kinase. Introduction of a ColEl hybrid plasmid containing the region into a strain with a chromosomal deletion of led to 3- and 17-fold increases in the specific activities of -glutamyl kinase and -glutamyl phosphate reductase, respectively.

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/content/journal/micro/10.1099/00221287-118-2-287
1980-06-01
2021-08-06
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