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Forty cysteine-requiring mutants of Paracoccus denitrificans were isolated. The growth requirements of these mutants were consistent with sulphite and sulphide being intermediates in the reduction of sulphate to cysteine in P. denitrificans. Fourteen were deficient in ATP sulphurylase and/or APS kinase. Of these, twelve were also unable to transport sulphate into the cell, showing that they were pleiotropic, being deficient in at least two and possibly three enzymes of the cysteine biosynthetic pathway.
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