1887

Abstract

Forty cysteine-requiring mutants of were isolated. The growth requirements of these mutants were consistent with sulphite and sulphide being intermediates in the reduction of sulphate to cysteine in . Fourteen were deficient in ATP sulphurylase and/or APS kinase. Of these, twelve were also unable to transport sulphate into the cell, showing that they were pleiotropic, being deficient in at least two and possibly three enzymes of the cysteine biosynthetic pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-1-79
1980-05-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/1/mic-118-1-79.html?itemId=/content/journal/micro/10.1099/00221287-118-1-79&mimeType=html&fmt=ahah

References

  1. Borum P. R., Monty K. J. 1976; Regulatory mutants and control of cysteine biosynthetic enzymes inSalmonella typhimurium.. Journal of Bacteriology 125:94–101
    [Google Scholar]
  2. Burnell J. N. 1975 Sulphur metabolism in Para- coccus denitrificans. D.Phil. thesis University of Oxford:
    [Google Scholar]
  3. Burnell J. N., Whatley F. R. 1977a; Sulphur metabolism inParacoccus denitrificans: purification, properties and regulation of serine trans- acetylase, O-acetylserine sulphydrylase and β- cystathionase.. Biochimica et biophysica acta 481:246–265
    [Google Scholar]
  4. Burnell J. N., Whatley F. R. 1977b; Sulphur metabolism inParacoccus denitrificans: purification, properties and regulation of cysteinyl- and methionyl-tRNA synthetases.. Biochimica et biophysica acta 481:266–278
    [Google Scholar]
  5. Burnell J. N., Whatley F. R. 1980; Regulation of sulphur metabolism inParacoccus denitrificans.. Journal of General Microbiology 118:73–78
    [Google Scholar]
  6. Burnell J. N., John P., Whatley F. R. 1975; The reversibility of active transport in membrane vesicles ofParacoccus denitrificans.. Biochemical Journal 150:527–536
    [Google Scholar]
  7. Clowes R. C. 1958; Nutritional studies of cysteineless mutants ofSalmonella typhimurium.. Journal of General Microbiology 18:140–153
    [Google Scholar]
  8. Collins J. M., Monty K. J. 1975; Cysteine biosynthesis inSalmonella typhimurium; the presence of ATP sulphurylase and APS kinase in various cysteine-requiring mutants.. Canadian Journal of Biochemistry 53:1118–1121
    [Google Scholar]
  9. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. 1969; Proposal to reject the genusHydrogenomonas: taxonomic implications.. International Journal of Systematic Bacteriology 19:375–390
    [Google Scholar]
  10. Dreyfuss J., Monty K. J. 1963; The biochemical characterization of cysteine-requiring mutants ofSalmonella typhimurium.. Journal of Biological Chemistry 238:1019–1024
    [Google Scholar]
  11. Jones-Mortimer M. C. 1968a; Positive control of sulphate reduction inEscherichia coli. Isolation, characterization and mapping of cysteineless mutants ofE. coli K12.. Biochemical Journal 110:589–595
    [Google Scholar]
  12. Jones-Mortimer M. C. 1968b; Positive control of sulphate reduction inEscherichia coli. The nature of the pleiotropic cysteineless mutants ofE. coliK12.. Biochemical Journal 110:597–602
    [Google Scholar]
  13. Kredich N. M. 1971; Regulation ofl-cysteine biosynthesis inSalmonella typhimurium : effects of growth on varying sulphur sources and O-acetyl- serine on gene expression.. Journal of Biological Chemistry 246:3474–3484
    [Google Scholar]
  14. Pasternak C. A. 1962; Control of sulphate activation inEscherichia coli andBacillus sub-tilis.. Biochemical Journal 85:44–49
    [Google Scholar]
  15. Postgate J. R. 1963; The examination of sulphur auxotrophs : a warning.. Journal of General Microbiology 30:481–484
    [Google Scholar]
  16. Robbins P. W., Lipmann F. 1958; Separation of the two enzymic phases in active sulphate synthesis.. Journal of Biological Chemistry 233:681–685
    [Google Scholar]
  17. Smith D. A. 1971; S-Amino acid metabolism and its regulation inEscherichia coli andSalmonella typhimurium.. Advances in Genetics 16:141–165
    [Google Scholar]
  18. Tully M., Yudkin M. D. 1977; Fine-structure mapping and complementation analysis of theEscherichia coli cysB gene.. Journal of Bacteriology 131:49–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-1-79
Loading
/content/journal/micro/10.1099/00221287-118-1-79
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error