1887

Abstract

(NCIB 7581) produced amylase throughout exponential growth and during the early-stationary phase. Enzyme synthesis occurred in the absence of -glucans but the yield was maximal when malt extract or starch was supplied as carbon source. Of the nitrogen sources examined, soya flour stimulated the highest yield of amylase. The enzyme was susceptible to reagents that react with thiol groups and had an exo-action on starch yielding maltose with a -anomeric configuration. It is concluded that the principal starch-hydrolysing enzyme from NCIB 7581 is a (1→4)---glucan maltohydrolase similar in its properties to other and plant -amylases.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-1-67
1980-05-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/1/mic-118-1-67.html?itemId=/content/journal/micro/10.1099/00221287-118-1-67&mimeType=html&fmt=ahah

References

  1. Claus D., Hunger W. 1980; Taxonomic studies on Bacillus megaterium and on agarolytic Bacillus strains.. In Classification and Identification of Aerobic Endospore-forming Bacteria. Berkeley R. C. W., Goodfellow M. Edited by London:: Academic Press.; (In the Press.)
    [Google Scholar]
  2. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for the determination of sugars and related substances.. Analytical Chemistry 28:350–356
    [Google Scholar]
  3. Fogarty W. M., Griffin P. J. 1975; Purification and properties of β-amylase produced by Bacillus polymyxa.. Journal of Applied Chemistry and Biotechnology 25:229–238
    [Google Scholar]
  4. French D. 1960; β-Amylases.. In The Enzymes 4A pp. 345–368 Boyer P. D., Lardy H., Myrback K. Edited by London & New York:: Academic Press.;
    [Google Scholar]
  5. Gibson T., Gordon R. E. 1974; Bacillus.. In Bergey’s Manual of Determinative Bacteriology, 8th. pp. 529–550 Buchanan R. E., Gibbons N. E. Edited by Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  6. Gordon R. E., Haynes W. C., Pang C.H.-N. 1973 The Genus Bacillus. Washington, D.C.:: United States Department of Agriculture.;
    [Google Scholar]
  7. Griffin P. J., Fogarty W. M. 1973; Production of an amylolytic enzyme by Bacillus polymyxa in batch cultures.. Journal of Applied Chemistry and Biotechnology 23:301–308
    [Google Scholar]
  8. Higashihara M., Okada S. 1974; Studies on α-amylase of Bacillus megaterium No. 32.. Agricultural and Biological Chemistry 38:1023–1029
    [Google Scholar]
  9. Logan N. A., Eerkeley R. C. W. 1980; Classification of the genus Bacillus based upon the AP1 system.. In Classification and Identification of Aerobic Endospore-forming Bacteria. Berkeley R. C. W., Goodfellow M. Edited by London:: Academic Press.; (In the Press.)
    [Google Scholar]
  10. Manners D. J. 1962; Enzymic synthesis and degradation of starch and glycogen.. Advances in Carbohydrate Chemistry 17:371–430
    [Google Scholar]
  11. Manners D. J., Stark J. R. 1974; α-(t-4)-d- glucans. Part XXII. The iodine staining properties of linear maltosaccharides.. Die Starke 26:78–81
    [Google Scholar]
  12. Miller G. L. 1959; Protein determination for large numbers of samples.. Analytical Chemistry 31:964–966
    [Google Scholar]
  13. Morgan F. J., Adams K. R., Priest F. G. 1979; A cultural method for the determination of pullulan-degrading enzymes in bacteria and its application to the genus Bacillus.. Journal of Applied Bacteriology 46:291–294
    [Google Scholar]
  14. Priest F. G. 1977; Extracellular enzyme synthesis in the genus Bacillus.. Bacteriological Reviews 41:711–753
    [Google Scholar]
  15. Priest F. G., Goodfellow M., Todd C. 1980; The genus Bacillus, a numerical analysis.. In Classification and Identification of Aerobic Endospore-forming Bacteria. Berkeley R. C. W., Goodfellow M. Edited by London:: Academic Press.; (In the Press.)
    [Google Scholar]
  16. Robyt J. F., Whelan W. J. 1968; The β- amylases.. In Starch and Its Derivatives, 4th. pp. 477–491 Radley J. A. Edited by London:: Chapman and Hall.;
    [Google Scholar]
  17. Shinke R., Kunimi Y., Nishira H. 1975a; Isolation and characterization of β-amylase producing micro-organisms.. Journal of Fermentation Technology 53:687–692
    [Google Scholar]
  18. Shinke R., Kunimi Y., Nishira H. 1975b; Production and some properties of β-amylases of Bacillus sp. BQ10.. Journal of Fermentation Technology 53:693–697
    [Google Scholar]
  19. Smith N. R., Gordon R. E., Clark F. E. 1952 Aerobic Spore-forming Bacteria. Washington, D.C.:: United States Department of Agriculture.;
    [Google Scholar]
  20. Takasaki Y. 1976a; Productions and utilizations of β-amylase and pullulanase from Bacillus cereusvar.mycoides.. Agricultural and Biological Chemistry 40:1515–1522
    [Google Scholar]
  21. Takasaki Y. 1976b; Purification and enzymatic properties of β-amylase and pullulanase from Bacillus cereus var.mycoides.. Agricultural and Biological Chemistry 40:1523–1530
    [Google Scholar]
  22. Trevelyan W. E., Procter D. P., Harrison J. S. 1951; Detection of sugars on paper chromatograms.. Nature London: 166:444–445
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-1-67
Loading
/content/journal/micro/10.1099/00221287-118-1-67
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error