1887

Abstract

A strain of (type I; Marples & McGinley, 1974 ), isolated from a blackhead acne lesion, produced an acid phosphatase which was present in the culture supernatant in the late-exponential and early-stationary phases of growth. This acid phosphatase was purified more than 45000-fold (4·5% yield). The purified enzyme gave two protein bands on sodium dodecyl sulphate-polyacrylamide gel electrophoresis corresponding to molecular weights of 155000 and 87100. The enzyme had a single peak of activity on Sephadex G-100, with a molecular weight corresponding to 93000. The highly purified acid phosphatase had an optimum activity at pH 5·8, was stable from pH 4·0 to 5·5 and was totally inactivated after 30 min at 55 °C. The enzyme did not show an absolute requirement for metal ions, but was stimulated by Mg, Ca, Zn and K at concentrations between 0·1 and 1 m The acid phosphatase was active against a number of monophosphate esters.

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/content/journal/micro/10.1099/00221287-118-1-59
1980-05-01
2021-05-18
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References

  1. Bessey O. A., Lowry O. H., Brock M. J. 1946; A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum.. Journal of Biological Chemistry 164:321–329
    [Google Scholar]
  2. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus.. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  3. Dole V. P., Meinertz H. 1960; Microdetermination of long chain fatty acids in plasma and tissues.. Journal of Biological Chemistry 235:2595–2599
    [Google Scholar]
  4. Hamilton W. A. 1975; Energy coupling in microbial transport.. Advances in Microbial Physiology 12:2–48
    [Google Scholar]
  5. Hoeffler U. 1977; Enzymatic and hemolytic properties of Propionibacterium acnes and related bacteria.. Journal of Clinical Microbiology 6:555–558
    [Google Scholar]
  6. Holland K. T., Cunliffe W. J., Roberts C. D. 1978; The role of bacteria in acne - a new approach.. Clinical and Experimental Dermatology 3:235–257
    [Google Scholar]
  7. Huggett A. St. G., Nixon D. A. 1957; Enzymic determination of blood glucose.. Biochemical Journal 66:12P
    [Google Scholar]
  8. Knüttila M. L. E., Mäkinen K. K. 1972; Purification and characterization of a phosphatase specifically hydrolysing p-nitrophenyl phosphate from an oral strain of Streptococcus mutans.. Archives of Biochemistry and Biophysics 152:685–701
    [Google Scholar]
  9. Malveaux F. J., San Clemente C. L. 1969; Staphylococcal acid phosphatase: extensive purification and characterisation of the loosely bound enzyme.. Journal of Bacteriology 97:1209–1214
    [Google Scholar]
  10. Marples R. R., McGinley K. J. 1974; Coryne-bacterium acnes and other anaerobic diphtheroids from human skin.. Journal of Medical Microbiology 7:349–357
    [Google Scholar]
  11. Millet J. 1970; Characterization of proteinases secreted by Bacillus subtilis Marburg strain during sporulation.. Journal of Applied Bacteriology 33:207–219
    [Google Scholar]
  12. Porschen R. K., Spaulding E. H. 1974; Phosphatase activity of anaerobic organisms.. Applied Microbiology 27:744–747
    [Google Scholar]
  13. Priest F. G. 1977; Extracellular enzyme synthesis in the genus Bacillus.. Bacteriological Reviews 41:711–753
    [Google Scholar]
  14. Puhvel S. M., Reisner M. D. 1972; The production of hyaluronidase (hyaluronate lyase) by Corynebacterium acnes.. Journal of Investigative Dermatology 58:66–70
    [Google Scholar]
  15. Reisner R. M., Silver D. Z., Puhvel S. M., Sternberg T. H. 1968; Lipolytic activity of Corynebacterium acnes.. Journal of Investigative Dermatology 51:190–196
    [Google Scholar]
  16. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for the estimation of N-acetyl amino sugars.. Journal of Biological Chemistry 217:959–966
    [Google Scholar]
  17. Weber K., Pringle J. R., Osborn M. 1972; Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.. Methods in Enzymology 26:3–27
    [Google Scholar]
  18. Weimberg R., Orton W. L. 1965; Elution of acid phosphatase from the cell surface of Saccharo-myces mellis by potassium chloride.. Journal of Bacteriology 90:82–94
    [Google Scholar]
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