Carbon Dioxide Fixation by Baker’s Yeast in a Variety of Growth Conditions Free

Abstract

Fixation of CO by growing under CO in a chemostat was investigated. Under anaerobic conditions, CO provided 6·5 ± 1% of the total carbon of yeast grown on glucose and 1·6% of the total carbon of yeast grown on glucose plus excess aspartate. Under aerobic conditions, 2·6% of the yeast carbon was derived from exogenous CO during growth on glucose or glycerol, and 3·3% during growth on pyruvate or ethanol.

The distribution of the fixed carbon among chemical components of the yeast, including some individual amino acids, was determined. Equilibration of CO across the cell membrane was probably not quite complete. Under anaerobic conditions, the similar molar radioactivities of aspartate and glutamate indicated that oxaloacetate was not metabolically compartmented. The unequal labelling of aspartate and glutamate during aerobic growth was consistent with operation of the glyoxylate bypass and/or compartmentation of oxaloacetate. Increased CO fixation and labelling of carbohydrate during growth on pyruvate or ethanol are ascribed to the activity of phospho pyruvate carboxykinase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-1-51
1980-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/1/mic-118-1-51.html?itemId=/content/journal/micro/10.1099/00221287-118-1-51&mimeType=html&fmt=ahah

References

  1. Burton K. 1956; Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochemical Journal 62:315–323
    [Google Scholar]
  2. Cannata J. J. B., Stoppani A. O. M. 1963; Phosphopyruvate carboxylase from baker’s yeast. Isolation, purification, and characterization.. Journal of Biological Chemistry 238:1196–1207
    [Google Scholar]
  3. Cazzulo J. J., Claissle M., Stoppani A. O. M. 1968; Carboxylase levels and carbon dioxide fixation in baker’s yeast.. Journal of Bacteriology 96:623–628
    [Google Scholar]
  4. Chapman C., Bartley W. 1968; The kinetics of enzyme changes in yeast under conditions that cause the loss of mitochondria.. Biochemical Journal 107:455–465
    [Google Scholar]
  5. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle.. Biochemical Journal 72:3P
    [Google Scholar]
  6. Duntze W., Neumann D., Gancedo J. M., Atzpodien W., Holzer H. 1969; Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. . European Journal of Biochemistry 10:83–89
    [Google Scholar]
  7. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  8. Gailiusis J., Rinne R. W., Benedict C. R. 1964; Pyruvate-oxaloacetate exchange reaction in baker’s yeast.. Biochimica et biophysica acta 92:595–601
    [Google Scholar]
  9. Haarasilta S., Oura E. 1975; On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of baker’s yeast.. European Journal of Biochemistry 52:1–7
    [Google Scholar]
  10. Haarasilta S., Taskinen L. 1977; Location of three key enzymes of gluconeogenesis in baker’s yeast.. Archives of Microbiology 113:159–161
    [Google Scholar]
  11. Heath D. F. 1968; The redistribution of carbon label by the reactions involved in glycolysis, gluconeogenesis and the tricarboxylic acid cycle in rat liver.. Biochemical Journal 110:313–335
    [Google Scholar]
  12. Liener I. E., Buchanan D. L. 1951; The fixation of carbon dioxide by growing and nongrowing yeast.. Journal of Bacteriology 61:527–534
    [Google Scholar]
  13. Machado A., Nuñez De Castro I., Mayor F. 1975; Isocitrate dehydrogenase and oxoglutarate dehydrogenase activities of baker’s yeast grown in a variety of hypoxic conditions.. Molecular and Cellular Biochemistry 6:93–100
    [Google Scholar]
  14. Mejbaum W. 1939; Über die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylsäure.. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 258:117–120
    [Google Scholar]
  15. Ochoa S. 1955; Malic dehydrogenase from pig heart.. Methods in Enzymology 1:735–739
    [Google Scholar]
  16. O’Leary M. H. 1976; Carbon isotope effect on the enzymatic decarboxylation of pyruvic acid.. Biochemical and Biophysical Research Communications 73:614–618
    [Google Scholar]
  17. Oura E. 1974a; Carbon dioxide fixation process in yeast, particularly baker’s yeast; theoretical background. Statement presented at the panel discussion, Production of yeast protein from different carbon sources, convenership H. W. D. Katinger. pp. 53–60 In Proceedings of the 4th International Symposium on Yeasts Vienna, part II pp. 58–59 Klaushofer H., Sleytr U.B. Vienna:
    [Google Scholar]
  18. Oura E. 1974b; Effect of aeration intensity on the biochemical composition of baker’s yeast. I. Factors affecting the type of metabolism.. Biotechnology and Bioengineering 16:1197–1212
    [Google Scholar]
  19. Pfäffli S., Suomalainen H. 1960; Determination of the carbohydrate fraction in baker’s yeast.. Suomen Kemistilehti 33B:61–65
    [Google Scholar]
  20. Rockwell G. E., Highberger J. H. 1927; The necessity of carbon dioxide for the growth of bacteria, yeasts and mold.. Journal of Infectious Diseases 40:438–446
    [Google Scholar]
  21. Ryle A. P., Sanger F., Smith L. F., Kitai R. 1955; The disulphide bonds of insulin.. Biochemical Journal 60:541–556
    [Google Scholar]
  22. Sorokin J. I. 1965; On the trophic role of chemo- synthesis and bacterial biosynthesis in water bodies.. Memorie dell’Istituto italiano di idrobiologia Dott. Marco de Marchi. Pallanza de Verbania Suppl. 18 169–205
    [Google Scholar]
  23. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids.. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  24. Strecker H. J., Ochoa S. 1954; Pyruvate oxidation system and acetoin formation.. Journal of Biological Chemistry 209:313–326
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-1-51
Loading
/content/journal/micro/10.1099/00221287-118-1-51
Loading

Data & Media loading...

Most cited Most Cited RSS feed