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Germination of Bacillus cereus 569 spores was triggered by inosine but not by adenosine. Germinated spores catabolized both inosine and adenosine. Addition of [U-14C]-labelled inosine or adenosine to the germination medium resulted in the release of 14CO2 which was dependent on exogenous phosphate and Mg2+. The released 14CO2 originated solely from the ribose moiety of either adenosine or inosine. The utilization of the ribose moiety of purine nucleosides supplied the energy needed for the removal of spore-specific photoproduct from DNA.
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