1887

Abstract

The effects of altered membrane sterol composition on the growth characteristics of sterol mutants of were determined using various energy sources and the detergent Tergitol. These mutants do not synthesize the end-product sterol, ergosterol, but do not require exogenous sterol for growth. The sterol biosynthetic intermediates that are incorporated into the mutant membranes are related to alterations in growth characteristics. The C-24 transmethylation step () was shown to be critical to membrane integrity. Cells with this lesion were protected by the presence of Tergitol or glycerol in the medium. A double mutant (), containing the Δ → Δ isomerization lesion () and the C-24 transmethylation lesion (), was highly sensitive to ethanol and Tergitol. These results corroborate permeability and membrane fluidity studies indicating that C sterols are much less efficient than C sterols in maintaining normal membrane structure and function.

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/content/journal/micro/10.1099/00221287-118-1-209
1980-05-01
2021-05-15
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References

  1. Bard M., Woods R. A., Barton D. H. R., Cor-rie J. E. T., Widdowson D. A. 1977; Sterol mutants of Saccharomyces cerevisiae: chromatographic analysis. Lipids 12:645–654
    [Google Scholar]
  2. Bard M., Lees N. D., Burrows L. S., Klein-Hans F. W. 1978; Differences in crystal violet uptake and cation-induced death among yeast sterol mutants. Journal of Bacteriology 135:1146–1148
    [Google Scholar]
  3. Barton D. H. R., Corrie J. E. T., Widdowson D. A., Bard M., Woods R. A. 1974; Biosynthesis of terpenes and steroids. Part IX. The sterols of some mutant yeasts and their relationship to the biosynthesis of ergosterol. Journal of the Chemical Society, Perkin Transactions I1326–1333
    [Google Scholar]
  4. Barton D. H. R., Gunatilaka A. A. L., Jarman T. R., Widdowson D. A., Bard M., Woods R. A. 1975; Biosynthesis of terpenes and steroids. Part X. The sterols of some yeast mutants doubly defective in ergosterol biosynthesis. Journal of the Chemical Society, Perkin Transactions I88–92
    [Google Scholar]
  5. Berliner L. J. editor 1976 Spin Labeling-Theory and Applications, 1st. New York:: Academic Press.;
    [Google Scholar]
  6. Cobon G. S., Haslam J. M. 1973; The effect of altered membrane sterol composition on the temperature dependence of yeast mitochondrial ATPase. Biochemical and Biophysical Research Communications 52:320–326
    [Google Scholar]
  7. Demel R. A., de Kruyff B. 1976; The function of sterols in membranes. Biochimica et biophysica acta 457:109–132
    [Google Scholar]
  8. Henry S. A., Keith A. D. 1971; Membrane properties of saturated fatty acid mutants of yeast revealed by spin labels. Chemistry and Physics of Lipids 7:245–265
    [Google Scholar]
  9. Hsia J. C., Schneider H. S., Smith I. C. P. 1971; A spin label study of the influence of cholesterol on phospholipid multibilayer structures. Canadian Journal of Biochemistry 49:614–622
    [Google Scholar]
  10. Kleinhans F. W., Lees N. D., Bard M., Haak R. A., Woods R. A. 1979; ESR determinations of membrane permeability in a yeast sterol mutant. Chemistry and Physics of Lipids 23:143–154
    [Google Scholar]
  11. Kores J., Oswald R., Keith A. 1972; Erythrocyte membranes-compression of lipid phases by increased cholesterol content. Biochimica et biophysica acta 274:71–74
    [Google Scholar]
  12. de Kruyff B. 1975; Lipid-sterol interactions in liposomes and membranes. Biochemical Society Transactions 3:618–621
    [Google Scholar]
  13. deKruyff B., Demel R. A., Van Deenen L. L. M. 1972; The effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact Acholeplasma laidlawii cell membranes and derived liposomes. Biochimica et biophysica acta 255:331–347
    [Google Scholar]
  14. Lees N. D., Bard M., Kemple M. D., Haak R. A., Kleinhans F. W. 1979; ESR determination of membrane order parameter in yeast sterol mutants. Biochimica et biophysica acta 553:469–475
    [Google Scholar]
  15. Molzhan S. W., Woods R. A. 1972; Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae. Journal of General Microbiology 72:339–348
    [Google Scholar]
  16. Parks L. W., McLean-Bowen C., Taylor F. R., Hough S. 1978; Sterols in yeast subcellular fractions. Lipids 13:730–735
    [Google Scholar]
  17. Pierce A. M., Pierce A. D. Jr Unrau A. M., Oehlschlager A. C. 1978; Lipid composition and polyene antibiotic resistance of Candida albicans mutants. Canadian Journal of Biochemistry 56:135–142
    [Google Scholar]
  18. Rottem S., Hubbell W. L., Hayflick L., McConnell H. M. 1970; Motion of fatty acid spin labels in the plasma membrane of Mycoplasma. Biochimica et biophysica acta 219:104–113
    [Google Scholar]
  19. Rottem S., Yashoav J., Neeman Z., Razin S. 1973; Cholesterol in Mycoplasma membranes. Composition, ultrastructure and biological properties of membranes from Mycoplasma mycoidesvar.capri cells adapted to grow with low cholesterol concentrations. Biochimica et biophysica acta 323:495–508
    [Google Scholar]
  20. Thompson E. D., Parks L. W. 1974; Effects of altered sterol composition on growth characteristics of Saccharomyces cerevisiae. Journal of Bacteriology 120:779–784
    [Google Scholar]
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