1887

Abstract

The biosynthesis by of candicidin, an aromatic polyene macrolide antibiotic, was inhibited by -tryptophan, -phenylalanine and, to a lesser degree, by -tyrosine. A mixture of the three aromatic amino acids inhibited candicidin biosynthesis to a greater extent than did each amino acid separately. -Tryptophan strongly inhibited the incorporation of the labelled precursors propionate or 4-aminobenzoic acid into candicidin. Incorporation of propionate into candicidin was 50 % inhibited by 2·5 m-tryptophan. Inhibition by tryptophan did not require protein synthesis as the same effect was observed in cells in which protein synthesis was prevented by chloramphenicol. The inhibitory effect of -tryptophan was partially reversed by exogenous 4-aminobenzoic acid suggesting that this effect is exerted at the level of 4-aminobenzoic acid synthase.

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1980-05-01
2021-07-31
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References

  1. Altendorf K. H., Gilch B., Lingens F. 1971; Biosynthesis of 4-aminobenzoic acid in Aerobacter aerogenes. FEBS Letters 16:95–98
    [Google Scholar]
  2. Francis M. M., Vining L. C., Westlake D. W. S. 1978; Characterization and regulation of anthranilate synthetase from a chloramphenicol-producing streptomycete. Journal of Bacteriology 134:10–16
    [Google Scholar]
  3. Gibson F., Pittard J. 1968; Pathways of biosynthesis of aromatic amino acids and vitamins and their control in microorganisms. Bacteriological Reviews 32:465–492
    [Google Scholar]
  4. Gibson F., Gibson M., Cox G. B. 1964; The biosynthesis of β-aminobenzoic acid from chorismic acid. Biochimica et biophysica acta 82:637–638
    [Google Scholar]
  5. Hagino H., Nakayama K. 1975; The biosynthetic control in aromatic amino acid producing mutants of Corynebacterium glutamicum. Agricultural and Biological Chemistry 39:351–361
    [Google Scholar]
  6. Huang M., Gibson F. 1970; Biosynthesis of 4-aminobenzoate in Escherichia coli. Journal of Bacteriology 102:767–773
    [Google Scholar]
  7. Jensen R. A., Rebello J. L. 1969; Comparative allostery of microbial enzymes at metabolic branch points: evolutionary implications. Developments in Industrial Microbiology 10:105–121
    [Google Scholar]
  8. Kane J. F., O’Brien H. D. 1975; p-Amino- benzoate synthase from Bacillus subtilis:amido-transferase composed of nonidentical subunits. Journal of Bacteriology 123:1131–1138
    [Google Scholar]
  9. Lingens F. 1976; Regulation of aromatic amino acid biosynthesis in microorganisms. Acta microbiologica Academiae scientiarum hungaricae 23:161–166
    [Google Scholar]
  10. Liu C. M., Mcdaniel L. L., Schaffner C. P. 1972; Studies on candicidin biogenesis. Journal of Antibiotics 25:116–121
    [Google Scholar]
  11. Martín J. F. 1977; Biosynthesis of polyene macrolide antibiotics. Annual Review of Microbiology 31:13–38
    [Google Scholar]
  12. Martín J. F., Demain A. L. 1976; Control by phosphate of candicidin production. Biochemical and Biophysical Research Communications 71:1103–1109
    [Google Scholar]
  13. Martín J. F., Liras P. 1976; Rapid incorporation of precursors into candicidin by resting cells of Streptomyces griseus. Journal of Antibiotics 29:1306–1309
    [Google Scholar]
  14. Martín J. F., Mcdaniel L. E. 1975a; Trophophase-idiophase transition in polyene macrolide antibiotics fermentations: cell maturation time. Biotechnology and Bioengineering 17:208–214
    [Google Scholar]
  15. Martín J. F., Mcdaniel L. E. 1975b; Specific inhibition of candicidin biosynthesis by the lysogenic inhibitor cerulenin. Biochimica et biophysica acta 411:186–194
    [Google Scholar]
  16. Martín J. F., Mcdaniel L. E. 1976a; Production of polyene macrolide antibiotics. Advances in Applied Microbiology 21:1–52
    [Google Scholar]
  17. Martín J. F., Mcdaniel L. E. 1976b; Biosynthesis of candicidin by phosphate limited resting cells of Streptomyces griseus. European Journal of Applied Microbiology 3:135–144
    [Google Scholar]
  18. Martín J. F., Liras P., Demain A. L. 1977; Inhibition by phosphate of the activity of candicidin synthases. FEMS Microbiology Letters 2:173–176
    [Google Scholar]
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