1887

Abstract

The base composition, DNA was found to be 62·2% GC, and the genome size was 2·1 × 10 daltons. There was no detectable difference between DNA from each of the three cell expressions examined. Reassociation kinetics indicated that no large group of repeated sequences was present, but that 5 % of the genome was composed of extremely rapidly reassociating sequences. No plasmids were detected. Electron microscopic examination showed that DNA contained short inverted repeat sequences on average 400 base pairs long. The possible function of these sequences is discussed.

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/content/journal/micro/10.1099/00221287-117-2-501
1980-04-01
2021-07-28
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References

  1. Barzilai R., Thomas C. A. Jr 1970; Spontaneous renaturation of newly synthesized bacteriophage T7 DNA.. Journal of MolecularBiology 51:145–155
    [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.. Gene 2:95–113
    [Google Scholar]
  3. Britten R. J., Kohne D. E. 1968; Repeated sequences in DNA.. science 161:529–540
    [Google Scholar]
  4. Britten R. J., Graham D. E., Neufeld B. R. 1974; Analysis of repeating DNA sequences by reassociation.. Methods in Enzymology 29:363–406
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA.. Biochemical Journal 62:315–323
    [Google Scholar]
  6. Clewell D. B., Helinski D. R. 1969; Super-coiled circular DNA-protein complex in Escherichia coli: purification induced conversion to open circular DNA form.. Proceedings of the National Academy of sciences of the United States of America 62:1159–1166
    [Google Scholar]
  7. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria.. Analytical Biochemistry 76:431–441
    [Google Scholar]
  8. Davidson E. H., Hough B. R., Amerson C. S., Britten R. J. 1973; General interspersion of repetitive with non-repetitive elements in the DNA of Xenopus.. Journal of Molecular Biology 77:1–23
    [Google Scholar]
  9. Davis R. W., Simon M., Davidson N. 1971; Electron microscopy heteroduplex methods for mapping regions of base sequence homology in nucleic acids.. Methods in Enzymology 21:413–428
    [Google Scholar]
  10. Dow C. S. 1972 Morphology and physiology of morphologically unusual bacteria. Ph.D. Thesis University of Warwick.:
    [Google Scholar]
  11. Dow C. S., France A. D. 1980; Simplified vegetative cell cycle of Rhodomicrobium vannielii.. Journal of General Microbiology 117:47–55
    [Google Scholar]
  12. Klotz L. C., Zimm B. H. 1972; Size of DNA determined by viscoelastic measurements: results in bacteriophages, Bacillus subtilis, and Escherichia coli.. Journal of Molecular Biology 72:779–800
    [Google Scholar]
  13. Kopecko D. J., Cohen S. N. 1975; Site specific recA independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.. Proceedings of the National Academy of sciences of the United States of America 72:1373–1377
    [Google Scholar]
  14. Mandel M., Leadbetter E. R., Pfennig N., Truper H. G. 1971; DNA base composition of phototrophic bacteria.. International Journal of Systematic Bacteriology 21:222–230
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature.. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  16. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.. Proceedings of the National Academy of sciences of the United States of America 57:1514–1521
    [Google Scholar]
  17. schmid C. W., Manning J. E., Davidson N. 1975; Inverted repeat sequences in the Drosophila genome.. Cell 5:159–172
    [Google Scholar]
  18. Staley J. T. 1968; Prosthecamicrobium and Ancala- microbium: new prosthecate freshwater bacteria.. Journal of Bacteriology 95:1921–1942
    [Google Scholar]
  19. Sutcliffe J. G. 1978; pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.. Nucleic Acids Research 5:2721–2728
    [Google Scholar]
  20. Whittenbury R., Dow C. S. 1977; Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria.. Bacteriological Reviews 41:754–808
    [Google Scholar]
  21. Wood N. B., Rake A. V., Shapiro L. 1976; Structure of Caulobacter DNA.. Journal of Bacteriology 126:1305–1315
    [Google Scholar]
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