1887

Abstract

Esterases of 14 strains of , 14 strains of , 16 strains of and 16 strains of were analysed by horizontal electrophoresis in polyacrylamide-agarose gel. Four principal esterase bands (designated E to E and nine minor bands differing in their activity towards synthetic substrates and in their sensitivity to heat and to di-isofluoropropyl phosphate were defined. The comparative distribution of bands showed that the four species analysed were characterized by distinct electrophoretic patterns of their esterases. Band E was found in all four species, bands E and E only in and band E only in some strains of The apparent molecular weights of esterases E and E, determined by electrophoresis in a 4 to 30 % polyacrylamide gradient gel, were 58000 (± 1000) and 72000 (±1800), respectively.

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/content/journal/micro/10.1099/00221287-117-2-483
1980-04-01
2021-10-16
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References

  1. Andersson L. O., Borg H., Mikaelsson M. 1972; Molecular weight estimations of proteins by electrophoresis in polyacrylamide gels of graded porosity.. FEES Letters 20:199–202
    [Google Scholar]
  2. Bascomb S., Lapage S. P., Willcox W. R., Curtis M. A. 1971; Numerical classification of the tribe Klebsielleae.. Journal of General Microbiology 66:279–295
    [Google Scholar]
  3. Brenner D. J., Farmer J. J., Hickman F. W., Asbury M. A., Steigerwalt A. G. 1977; Taxonomic and nomenclature changes in Enterobacteriaceae.. Center for Disease Control, Department of Health, Education and Welfare, Publication No. 78–8356. Atlanta, Georgia, U.S.A.: C.D.C.;
    [Google Scholar]
  4. Buchanan R. E., Gibbons N. E.editors 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Gavini F., Leclerc H., Lefebvre B., Ferragut C., Izard D. 1977; Etude taxonomique d’Enterobactéries appartenant ou apparentées au genre Klebsiella.. Annales de Microbiologie 128B:45–49
    [Google Scholar]
  6. Gornall A. G., Bardawill D. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction.. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  7. Goullet Ph. 1975; Esterase zymograms of Proteus and Providencia.. Journal of General Microbiology 87:97–106
    [Google Scholar]
  8. Goullet Ph. 1977; Relationships between electrophoretic patterns of esterases from Salmonella.. Journal of General Microbiology 98:535–542
    [Google Scholar]
  9. Hugh R. 1959; Oxytoca group organisms isolated from the oropharyngeal region.. Canadian Journal of Microbiology 5:251–254
    [Google Scholar]
  10. Jain K., Radsak K., Mannheim W. 1974; Differentiation of the Oxytocum group from Klebsiella by deoxyribonucleic acid-deoxyribonucleic acid hybridization.. International Journal of Systematic Bacteriology 24:402–407
    [Google Scholar]
  11. Johnson R., Colwell R. R., Sakazaki R., Tamura K. 1975; Numerical taxonomy study of the Enterobacteriaceae.. International Journal of Systematic Bacteriology 25:12–37
    [Google Scholar]
  12. Lautrop H. 1956; Gelatin-liquefying Klebsiellastrains (Bacterium oxytocum (Flüggel)).. Acta pathologica et microbiologica scandinavica 39:375–384
    [Google Scholar]
  13. Margolis J., Kenrick K. G. 1968; Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient.. Analytical Biochemistry 25:347–362
    [Google Scholar]
  14. Orskov I. 1974; Genus VI Klebsiella Trevisan 1885.. In Bergey’s Manual of Determinative Bacteriology, 8th edn.. pp. 321–324 Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Richard C. 1973; Etude antigénique et biochimique de 500 souches de Klebsiella.. Annales de biologie clinique 31:295–303
    [Google Scholar]
  16. Richard C. 1977; Présence chez Enterobacter aerogenes d’antigènes capsulairés apparentes à ceux de Klebsiella: intèrêt de l’utilisation du métahydroxybenzoate dans le diagnostic différentiel E. aerogenes - K. pneumoniae.. Annales de Microbiologie 128A:289–295
    [Google Scholar]
  17. Richard C. 1979; Entérobactéries inhabituelles.. Bulletin de l’Institut Pasteur 77:83–98
    [Google Scholar]
  18. Richard C., Joly B., Sirot J., Stoleru G. H., Popoff M. 1976; Etude de souches d’Entero-bacter appartenant à un groupe particulier proche de E. aerogenes.. Annales de Microbiologie 127A:545–548
    [Google Scholar]
  19. Rodbard D., Kapadia G., Chrambach A. 1971; Pore gradient electrophoresis.. Analytical Biochemistry 40:135–157
    [Google Scholar]
  20. Steffen D. L., Cocks G. T., Wilson A. C. 1972; Micro-complement fixation in Klebsiellaclassification.. Journal of Bacteriology 110:803–808
    [Google Scholar]
  21. Von Riesen V. L. 1976; Pectinolytic, indolepositive strains of Klebsiella pneumoniae.. International Journal of Systematic Bacteriology 26:143–145
    [Google Scholar]
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