Isolation and Characterization of a Mutant of K12 Synthesizing DNA Polymerase I and Endonuclease I Constitutively Free

Abstract

A mutant of K12, highly resistant to ultraviolet radiation, has been isolated. Preliminary tests show that this mutant is also resistant to mitomycin C, nalidixic acid, fluorouracil and thymineless death. The mutant strain apparently repairs its damaged DNA more efficiently than wild-type K12 strains and, to do so, constitutively produces 35 times more DNA polymerase I and 12 times more endonuclease I than the wild-type strain.

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1980-04-01
2024-03-29
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