1887

Abstract

A spectrofluorometric assay for the estimation of the tremorgenic mycotoxin verruculogen in crude mycelial extract has been devised and used to determine concentrations as low as 0·2 g ml. Verruculogen production by has been extended from surface culture to submerged culture in 60 1 stirred fermenters, in which the maximum cell-associated mycotoxin yield [5 mg (100 ml culture)] was obtained within 7 d. It was found necessary to supplement the medium (Czapek Dox broth plus 0·5 % yeast extract) with calcium chloride (2 %) to induce profuse sporulation (2 × 10 conidia ml).

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/content/journal/micro/10.1099/00221287-117-2-405
1980-04-01
2022-01-27
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