1887

Abstract

Five strains of a new alkalophilic bacterium have been isolated from potato-processing effluent. These strains are Gram-positive, non-sporing, motile rods which form an orange, cell-bound pigment and are capable of growth in aerobic or anaerobic conditions at a pH up to 11·5 and between 7 and 43°C. For one representative strain the highest growth rate occurred in the pH range 8 to 10·5 and the minimum doubling time observed was 27 min at approximately 38°C. With glucose as substrate, lactate, acetate and formate were major end-products, the proportions depending on cultural conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-1-9
1980-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/1/mic-117-1-9.html?itemId=/content/journal/micro/10.1099/00221287-117-1-9&mimeType=html&fmt=ahah

References

  1. Akiba T., Horikoshi K. 1976; Identification and growth characteristics of α-galactosidase-producing microorganisms. Agricultural and Biological Chemistry 40:1845–1849
    [Google Scholar]
  2. Barnes E. M., Impey C. S. 1972; Some properties of the nonsporing anaerobes from poultry caeca. Journal of Applied Bacteriology 35:241–251
    [Google Scholar]
  3. Bornside G. H., Kallio R. E. 1956; Ureahydrolyzing bacilli I. A physiological approach to identification. Journal of Bacteriology 71:627–634
    [Google Scholar]
  4. Boyer E. W., Ingle M. B., Mercer G. D. 1973; Bacillus alcalophilus subsp.halodurans subsp.nov., an alkaline-amylase-producing al-kalophilic organism. International Journal of Systematic Bacteriology 23:238–242
    [Google Scholar]
  5. Chislett M. E., Kushner E>.J. 1961; A strain of Bacillus circulans capable of growing under highly alkaline conditions. Journal of General Microbiology 24:187–190
    [Google Scholar]
  6. Cowan S. T. 1974 Cowan & Steel’s Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  7. Crombach W. H. J. 1978; DNA base ratios and DNA hybridisation studies of coryneform bacteria, mycobacteria and nocardiae. In Coryneform Bacteria pp. 161–179 Bousfield I. J., Callely A. G. Edited by London: Academic Press;
    [Google Scholar]
  8. Cruickshank R.editor 1965 Medical Microbiology, 11th edn. Edinburgh: E. & S. Livingstone;
    [Google Scholar]
  9. Elsden S. R., Gibson Q. H. 1954; The estimation of lactic acid using ceric sulphate. Biochemical Journal 58:154–158
    [Google Scholar]
  10. Grant W. D., Mills A. A., Schofield A. K. 1979; An alkalophilic species of Ectothiorhodospira from a Kenyan soda lake. Journal of General Microbiology 110:137–142
    [Google Scholar]
  11. Guffanti A. A., Susman P., Blanco R., Krulwich T. A. 1978; The protonmotive force and α-aminoisobutyric acid transport in an obligately alkalophilic bacterium. Journal of Biological Chemistry 253:708–715
    [Google Scholar]
  12. Hale E. M. 1977; Isolation of alkalophilic bacteria from an alkaline reservoir used in an industrial process. Proceedings of the 11th Annual Meeting of the American Society for Microbiology abstract 150
    [Google Scholar]
  13. Hankin L., Anagnostakis S. L. 1977; Solid media containing carboxymethylcellulose to detect C1 cellulase activity of micro-organisms. Journal of General Microbiology 98:109–115
    [Google Scholar]
  14. Hankin L., Zucker M., Sands D. C. 1971; Improved solid medium for the detection and enumeration of pectolytic bacteria. Applied Microbiology 22:205–209
    [Google Scholar]
  15. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gramnegative bacteria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  16. Ikura Y., Horikoshi K. 1977; Isolation and some properties of alkalophilic bacteria utilizing rayon waste. Agricultural and Biological Chemistry 41:1373–1377
    [Google Scholar]
  17. Jones D. W., Kay J. J. 1976; Determination of volatile fatty acids C1-C8 and lactic acid in silage juice. Journal of the Science of Food and Agriculture 27:1005–1014
    [Google Scholar]
  18. Jones D., Watkins J., Erickson S. K. 1973; Taxonomically significant colour changes in Brevibacterium linens probably associated with a carotenoid-like pigment. Journal of General Microbiology 77:145–150
    [Google Scholar]
  19. Kitada M., Horikoshi K. 1976; Alkaline protease production from methyl acetate by alkalophilic Bacillus sp. Journal of Fermentation Technology 54:383–392
    [Google Scholar]
  20. Markham R. 1942; A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochemical Journal 36:790–791
    [Google Scholar]
  21. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  22. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  23. Matches J. R., Liston J. 1973; Temperature-gradient incubator for the growth of clostridia. Canadian Journal of Microbiology 19:1161–1165
    [Google Scholar]
  24. Mencier F. 1972; Méthode simple et rapide de mise en évidence des microorganismes producteurs de dextranase. Annales de l’Institut Pasteur 122:153–157
    [Google Scholar]
  25. Nakamura N., Horikoshi K. 1976; Characterization and some cultural conditions of a cyclodextrin glycosyltransferase-producing alkalophilic Bacillus sp. Agricultural and Biological Chemistry 40:753–757
    [Google Scholar]
  26. Ohta K., Kiyomiya A., Koyama N., Nosoh Y. 1975; The basis of the alkalophilic property of a species of Bacillus. Journal of General Microbiology 86:259–266
    [Google Scholar]
  27. Souza K. A., Deal P. H. 1977; Characterization of a novel extremely alkalophilic bacterium. Journal of General Microbiology 101:103–109
    [Google Scholar]
  28. Souza K. A., Deal P. H., Mack H. M., Turnbill C. E. 1974; Growth and reproduction of microorganisms under extremely alkaline conditions. Applied Microbiology 28:1066–1068
    [Google Scholar]
  29. Sunaga T., Akiba T., Horikoshi K. 1976; Production of penicillinase by an alkalophilic Bacillus. Agricultural and Biological Chemistry 40:1363–1367
    [Google Scholar]
  30. Vedder A. 1934; Bacillus alcalophilus n.sp. Antonie van Leeuwenhoek 1:141–147
    [Google Scholar]
  31. Werner W., Rey H. G., Wielinger H. 1970; Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/ POD-Methode. Zeitschrift für analytische Chemie 252:224–228
    [Google Scholar]
  32. Wiley W. R., Stokes J. L. 1962; Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. Journal of Bacteriology 84:730–734
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-1-9
Loading
/content/journal/micro/10.1099/00221287-117-1-9
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error