Summary: Cyanide production by growing on a -glutamate/minimal salts medium was stimulated by addition of 2 m-glycine and 0·5 m-methionine to the medium. However, when the initial concentration of glycine was 0·6 m, a lower level of cyanogenesis was obtained than that with glycine-free medium. Cystathionine, -methionine sulphoxide, -methionine and -adenosyl--homocysteine were effective substitutes for -methionine, but a wide range of other compounds including betaine and choline were inactive. Cyanogenesis was stimulated when threonine was used as a replacement for glycine, but serine and a range of analogues of glycine were ineffective. In a minimal salts medium containing glucose as the carbon source, glycine or NHCl but not methionine could serve as the sole nitrogen source for growth. Growth on glucose plus NHCl in the presence of glycine and methionine resulted in greater yields of cyanide than growth on a similar medium in the absence of NHCl. In the latter case raising the initial concentration of glycine, to compensate for its utilization as the sole source of nitrogen, had little effect on cyanogenesis. Addition of cyclic AMP to both the NHCl-containing and NHCl-free media caused a decrease in cyanide formation.


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