1887

Abstract

The effects of the following changes throughout the association of germ-free mice with increasing numbers of anaerobic bacteria were studied: (i) elution patterns obtained by gel-filtration chromatography of caecal diffusates; (ii) concentration of -aspartylglycine in caecal and faecal contents; (iii) polypeptide patterns obtained by sodium dodecyl sulphatepolyacrylamide gel electrophoresis of caecal supernatants; (iv) free amino acid content of caecal supernatants; (v) faecal bile acids, analysed by gas-liquid chromatography; (vi) colonization-resistance. The results indicate that monitoring the normalization (association) process can be accomplished in several ways, but the level of colonization-resistance is most easily measured by high-voltage paper electrophoresis of faecal supernatants to determine the concentration of -aspartylglycine. During association, the concentration of -aspartyl-glycine decreased and became undetectable after association with 40 to 50 different strains of bacteria. There was a good negative correlation between the level of colonization-resistance and the concentration of -aspartylglycine.

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/content/journal/micro/10.1099/00221287-117-1-57
1980-03-01
2021-05-15
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References

  1. Aries V. C., Hill M. J. 1970; Degradation of steroids by intestinal bacteria. 11. Enzymes catalysing the oxidoreduction of the 3α-, 7α- and 12α-hydroxylgroups in cholic acid, and the dehydroxylation of the 7α-hydroxylgroup. Biochimica et biophysicn acta 202:535–543
    [Google Scholar]
  2. Goddard P., Hill M. J. 1972; Degradation of steroids by intestinal bacteria. IV. The aromatisation of ring A. Biochimica et biophysica acta 280:336–342
    [Google Scholar]
  3. Goddardp P., Fernandez F., West B., Hill M. J., Barnesp P. 1975; The nuclear dehydrogenation of steroids by intestinal bacteria. Juurncil of Medical Microbiolog 8:429–435
    [Google Scholar]
  4. Koopman J. P., Janssen F. G. J., Van Dkuten J. A. M. 1977; The relation between the intestinal microflora and intestinal parameters in mice. Zeitschrift für Versuchstierkunde 9:54–61
    [Google Scholar]
  5. Laemmliu U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  6. Macdonald I. A., Meiere E. C., Mahonyd D. E., Costaing G. A. 1976; 3α-, 7α- and 12α-hydroxysteroid dehydrogenase activities fromClostridium perjringens . Biochimica et biophysica acta 450:142–153
    [Google Scholar]
  7. Naughton M. A., Sanger F., Hartley B. S., Shaw D. C. 1960; The amino acid sequence around the reactive serine residue of some proteolytic enzymes. Biochemical Journal 77:149–163
    [Google Scholar]
  8. Van der waaij D., Berghuis J. M. 1974; Determination of the colonization resistance of the digestive tract of individual mice. Journal of Hygiene 72:379–387
    [Google Scholar]
  9. Van der Waaij D., Berghuis-de Vries J. M., Lekkerkerk-Van der Wees J. E. C. 1971; Colonization resistance of the digestive tract in conventional and antibiotic-treated mice. Journal of Hygiene 69:405–411
    [Google Scholar]
  10. Welling G. W. 1979; β-Aspartylglycine, an indicator of decreased colonization-resistance?. In New Criteria for Antimicrobial Therapy pp. 65–71 van der Waaij D., Verhoef J. Edited by Amsterdam: Excerpta Medica;
    [Google Scholar]
  11. Welling G. W., Groen G. 1978; β-Aspartyl- glycine, a substance unique to caecal contents of germ-free and antibiotic-treated mice. Biochemical Journal 175:807–812
    [Google Scholar]
  12. Wensinck F., Ruseler-van Embden J. G. H. 1971; The intestinal flora of colonization-resistant mice. Journal of Hygiene 69:413–421
    [Google Scholar]
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