%0 Journal Article %A Garber, Nachman %A Nachshon, Israela %T Localization of Cholinesterase in Pseudomonas aeruginosa Strain K %D 1980 %J Microbiology, %V 117 %N 1 %P 279-283 %@ 1465-2080 %R https://doi.org/10.1099/00221287-117-1-279 %I Microbiology Society, %X The inducible cholinesterase of Pseudomonas aeruginosa strain K (ATCC 25102) degraded propionylcholine, acetylthiocholine, acetylcholine and acetyl-β-methylcholine at a high rate and butyrylcholine and succinylcholine at very low rates. The localization of the enzyme in the periplasmic space was indicated by a similar rate of acetylcholine degradation by intact cells or their extracts, by release of cholinesterase together with alkaline phosphatase into the culture medium during cell growth in a low phosphate-containing medium, by liberation of cholinesterase and alkaline phosphatase during lysozyme-induced conversion of cells to spheroplasts and by freezing and thawing. Treatment of cells with diazo-7-amino-1,3-naphthalenedisulphonic acid, which inactivates surface-located enzymes, abolished most of the cholinesterase and 5′-nucleotidase activities. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-117-1-279