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Summary: A mutant (CD11) of Vibrio cholerae was isolated after exposure of a wild-type pathogenic strain (KB207) to N-methyl-N'-nitro-N-nitrosoguanidine. Although the mutant was motile and chemotactic, it adhered poorly to rabbit intestinal mucosa. Pretreatment of vibrios with 10 mg d-mannose mh−1 and of intestinal mucosa with 100 mmol sodium metaperiodate inhibited adherence of KB207 but not of CD11. The gene (ams) controlling mannose-sensitive adherence was mapped on the bacterial chromosome closely linked to the pur locus.