Summary: The frequency of transposition of Tn to pGC200, an IncFII R plasmid, increased during the storage of the host strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30°C but it was nearly undetectable when the host strain was grown and kept at 37°C. Fusions between two different plasmids carrying Tn with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tn


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