1887

Abstract

NCIB 8639 utilized a number of cellulosic substrates and produced extracellular carboxymethylcellulase and activity towards Avicel (a milled micro-crystalline cellulose powder). Both types of enzyme were synthesized in media containing glucose but the activity was influenced by the nature and concentration of the carbon source. The ratio of carboxymethylcellulase activities measured by reducing sugar production and by viscosity decrease was also influenced by the nature of the cellulosic substrate supplied to cultures. The greatest extracellular enzyme production occurred in cultures grown on microcrystalline cellulose powders such as Avicel and Whatman CC31 at concentrations up to 4% (w/v). The greatest degradation rate observed was in cultures supplied with Avicel where 60 % of the substrate was degraded in 4 d.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-1-235
1980-03-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/1/mic-117-1-235.html?itemId=/content/journal/micro/10.1099/00221287-117-1-235&mimeType=html&fmt=ahah

References

  1. Almin K. E., Eriksson K. E. 1967; Enzymatic degradation of polymers. I. Viscometric method for the determination of enzymatic activity. Biochimica et biophysica acta 139:238–247
    [Google Scholar]
  2. Almin K. E., Eriksson K. E. 1968; Influence of carboxymethylcellulose properties on the determination of cellulase activity in absolute terms. Archives of Biochemistry and Biophysics 124:129–134
    [Google Scholar]
  3. Almin K. E., Eriksson K. E. 1975; Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum for the breakdown of cellulose. European Journal of Biochemistry 51:207–211
    [Google Scholar]
  4. Berg B. v Hofsten B., Pettersson G. 1972a; Growth and cellulase formation by Cellvibrio fulvus . Journal of Applied Bacteriology 35:201–214
    [Google Scholar]
  5. Berg B. v Hofsten B., Pettersson G. 1972b; Electron microscopic observations on the degradation of cellulose fibres by Cellvibrio fulvus and Sporocytophaga myxococcoides . Journal of Applied Bacteriology 35:215–219
    [Google Scholar]
  6. Chang W., Thayer D. W. 1977; Thecellulase system of a Cytophaga species. Canadian Journal of Microbiology 23:1285–1292
    [Google Scholar]
  7. Dubois M., Gilles K., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  8. Fahraeus G. 1947; Studies in the cellulose decomposition by Cytophaga . Symbolae botanicae upsalienses 9:1–128
    [Google Scholar]
  9. Halliwell G., Griffin M. 1978; Affinity chromatography of the cellulase system of Trichoderma koningii . Biochemical Journal 169:713–715
    [Google Scholar]
  10. v Hofsten B. 1975; Topological effects in enzymatic and microbial degradation of highly ordered polysaccharides. In Proceedings of the Symposium on Enzymatic Hydrolysis of Cellulose pp. 281–295 Bailey M., Enary T. M., Linko M. Edited by Helsinki: S.I.T.R.A;
    [Google Scholar]
  11. Hulme M. A. 1971; Viscometric determination of carboxymethylcellulase in standard international units. Archives of Biochemistry and Biophysics 147:49–54
    [Google Scholar]
  12. Kaufmann A., Fegan J., Doleac P., Gainer C., Wittich D., Glann A. 1976; Identification and characterization of a cellulolytic isolate. Journal of General Microbiology 94:405–408
    [Google Scholar]
  13. Mandels M., Weber J. 1969; The production of cellulases. In Cellulases and Their Applications, Advances in Chemistry Series 95 pp. 391–413 Washington: American Chemical Society;
    [Google Scholar]
  14. Miller G. L. 1959; Use of dinitrosalicylic acid reagent for the determination of reducing sugar. Analytical Chemistry 31:426–428
    [Google Scholar]
  15. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  16. Osmundsvag K., Goksøyr J. 1975; Cellulases from Sporocytophaga myxococcoides, purification and properties. European Journal of Biochemistry 57:405–409
    [Google Scholar]
  17. Reese E. T., Maguire A. 1969; Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiology 17:242–245
    [Google Scholar]
  18. Sternberg D., Dorval S. 1979; Cellulase production and ammonia metabolism in Trichodermareesei on high levels of cellulose. Biotechnology and Bioengineering 21:181–191
    [Google Scholar]
  19. Suzuki H., Yamane K., Nisizawa K. 1969; Extracellular and cell-bound cellulase components of bacteria. In Cellulases and Their Applications,Advances in Chemistry Series 95 pp. 60–82 Washington: American Chemical Society;
    [Google Scholar]
  20. Szajer C., Targonski Z. 1977; Lignocellulose fermentations. In Biotechnology and Fungal Differentiation Meyrath J., Bu’Lock J. D. Edited by London: Academic Press;
    [Google Scholar]
  21. Tampion J. 1965; New method for the determination of the activity of viscosity reducing enzymes. Nature; London: 206:510–511
    [Google Scholar]
  22. Vance I., Stanley S. O., Brown C. M. 1979; A microscopical investigation of the bacterial degradation of wood pulp in a simulated marine environment. Journal of General Microbiology 114:69–74
    [Google Scholar]
  23. Wood T. M., Mccrae S. I. 1978; The cellulase of Trichoderma koningii . Biochemical Journal 171:61–72
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-1-235
Loading
/content/journal/micro/10.1099/00221287-117-1-235
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error