Phenotypic and Genotypic Characterization of Some Thermophilic Species of Free

Abstract

Some thermophilic species of were characterized using biochemical tests, antibiotic sensitivity, bacteriocin and bacteriophage sensitivity, esterase patterns, DNA hybridization and % G+C content. The three caldo-active strains of bacilli isolated by Heinen (1971) were compared with strains of Eight of the strains examined showed characteristics which enabled them to be placed in the three main taxonomic groups suggested by Walker & Wolf (1971) . Two strains showed characteristics of both groups 1 and 3. Examination of genotype data showed taxonomic groupings which differed from those based on phenotypic characterization.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-1-201
1980-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/1/mic-117-1-201.html?itemId=/content/journal/micro/10.1099/00221287-117-1-201&mimeType=html&fmt=ahah

References

  1. Atkinson A. 1973; The purification of microbial enzymes. Process Biochemistry 8(8):9–12
    [Google Scholar]
  2. Atkinson A., Evans C. G. T., Yeo R. G. 1975; Behaviour of Bacillus stearothermophilus grown in different media. Journal of Applied Bacteriology 38:301–304
    [Google Scholar]
  3. Baillie A., Norris J. R. 1963; Studies on enzyme changes during sporulation in Bacillus cereus using starch gel electrophoresis. Journal of Applied Bacteriology 26:102–108
    [Google Scholar]
  4. Baillie A., Walker P. D. 1968; Enzymes of thermophilic aerobic spore-forming bacteria. Journal of Applied Bacteriology 31:114–119
    [Google Scholar]
  5. Bingham A. H. A., Atkinson A., Sciaky D., Roberts R. 1978; A specific endonuclease from Bacillus caldolyticus . Nucleic Acids Research 5:3457–3467
    [Google Scholar]
  6. Brock T. D., Freeze H. 1969; Thermus aquaticus, a non-sporulating extreme thermophile. Journal of Bacteriology 98:289–297
    [Google Scholar]
  7. Buchanan R. E., Gibbons N. E. editors 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Cazzulo J. J., Sundaram T. K., Kornberg H. L. 1969; Regulation of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Nature; London: 223:1137–1140
    [Google Scholar]
  9. Cazzulo J. J., Sundaram T. K., Kornberg H. L. 1970; Mechanism of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Nature; London: 227:1103–1105
    [Google Scholar]
  10. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  11. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–652
    [Google Scholar]
  12. Epstein I., Grossowicz N. J. 1969; Prototrophic thermophilic bacillus: isolation, properties and kinetics of growth. Journal of Bacteriology 99:414–417
    [Google Scholar]
  13. Heinen W. 1971; Growth conditions and temperature-dependent substrate specificity of two extremely thermophilic bacteria. Archiv für Mikrobiologie 76:2–17
    [Google Scholar]
  14. Heinen U. J., Heinen W. 1972; Characteristics and properties of a caldo-active bacterium producing extracellular enzymes and two related strains. Archiv für Mikrobiologie 82:1–23
    [Google Scholar]
  15. Jung L., Jost R., Stoll E., Zuber H. 1974; Metabolic differences in B. stearothermophilus grown at 55 °C and 37 °C. Archives of Microbiology 95:125–138
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–220
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  18. Sargeant K., East D. N., Whitaker A. R., Elsworth R. 1971; Production of Bacillus stearothermophilus NCA 1503 for glyceraldehyde-3-phosphate dehydrogenase. Journal of General Microbiology 65:iii
    [Google Scholar]
  19. Shah H. N., Williams R. A. D., Bowden G. H., Hardie J. M. 1976; Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. Journal of Applied Bacteriology 41:473–492
    [Google Scholar]
  20. Sharp R. J., Comer M. J., Bingham A. H. A., Atkinson A. 1979; Partial characterization of a bacteriocin (thermocin) from Bacillus stearothermophilus RS93. Journal of General Microbiology 111:449–451
    [Google Scholar]
  21. Singleton R., Amelunxen R. E. 1973; Proteins from thermophilic microorganisms. Bacteriological Reviews 37:320–342
    [Google Scholar]
  22. Walker P. D., Wolf J. 1971; The taxonomy of Bacillus stearothermophilus . In Spore Research pp. 247–264 Edited by Barker A. N., Gould G. W., Wolf J. London: Academic Press;
    [Google Scholar]
  23. Williams R. A. D. 1975; Caldoactive and thermophilic bacteria and their thermostable proteins. Science Progress 62:373–393
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-1-201
Loading
/content/journal/micro/10.1099/00221287-117-1-201
Loading

Data & Media loading...

Most cited Most Cited RSS feed