1887

Abstract

A poly(l,4-α--galacturonide) lyase (EC 4.2.2.2) from the culture fluid of was purified about 20-fold. The optimum pH and temperature for enzyme activity were 8·0 and 40C. The enzyme required Ca and was inhibited by EDTA; it preferred polygalacturonate as substrate, cleaving 1,4-α-glycosidic linkages randomly to form unsaturated galacturonates, mainly the unsaturated digalacturonate. Some properties of the crude and purified enzyme preparations are described. An exopolygalacturonase is also produced by this organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-1-193
1980-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/1/mic-117-1-193.html?itemId=/content/journal/micro/10.1099/00221287-117-1-193&mimeType=html&fmt=ahah

References

  1. Bryant M. P., Small P. 1956; Characteristics of two genera of anaerobic curved rods isolated from the rumen of cattle. Journal of Bacteriology 72:22–26
    [Google Scholar]
  2. Clarke R. T. J., Bailey R. W., Gaillard B. D. E. 1969; Growth of rumen bacteria on plant cell wall polysaccharides. Journal of General Microbiology 56:79–86
    [Google Scholar]
  3. Dehority B. A. 1969; Pectin fermenting bacteria isolated from the bovine rumen. Journal of Bacteriology 99:189–196
    [Google Scholar]
  4. Gee M., Mccready R. M. 1957; Paper chromatographic detection of galacturonic acid and glucuronic acids. Analytical Chemistry 29:257–258
    [Google Scholar]
  5. Gradel C. M., Dehority B. A. 1972; Fermentation of isolated pectin and pectin from intact forages by pure cultures of rumen bacteria. Applied Microbiology 23:332–340
    [Google Scholar]
  6. Hasegawa S., Nagel C. W. 1967; Separation of a hydrolase from a pectic acid transeliminase in cell extracts of a Bacillus. Nature; London: 213207–209
    [Google Scholar]
  7. Hatanaka C., Ozawa J. 1971; Enzymic degradation of pectic acid. XIII. A new exopolygalacturonase producing digalacturonic acid from pectic acid. Berichte des Ohara Instituts für landwirtschaftliche Biologie 15:47–60
    [Google Scholar]
  8. Heinrichova K., Rexova-Benkova L. 1976; Purification and characterization of an extracellular exo-D-galacturonase of Aspergillus niger. . Biochimica and biophysica acta 422:349–356
    [Google Scholar]
  9. Kamimiya S., Itoh Y., Izaki K., Takahashi H. 1977; Purification and properties of a pectate lyase in Erwinia aroideae . Agricultural and Biological Chemistry 41:975–981
    [Google Scholar]
  10. Kapitonova L. S., Rodionova N. A., Fenixova R. V. 1972; Pectolytic enzymes of Clostridium felsineum. . Prikladnaya biokhimiya i mikrobiologiya 8:539–545
    [Google Scholar]
  11. Kapitonova L. S., Rodionova N. S., Fenixova R. V. 1973; Purification and characterization of transeliminase of pectic acid of Clostridium felsineum . Biokhimiya 38:1054–1061
    [Google Scholar]
  12. Lanning M. C., Cohen S. S. 1951; The detection and estimation of 2-ketohexuronic acids. Journal of Biological Chemistry 189:109–114
    [Google Scholar]
  13. Lund B. M., Brocklehurst T. F. 1978; Pectic enzymes of pigmented strains of Clostridium. . Journal of General Microbiology 104:59–66
    [Google Scholar]
  14. Nagel C. W., Vaughn R. H. 1961; The degradation of oligogalacturonides by the polygalacturonase of Bacillus polymyxa . Archives of Biochemistry and Biophysics 94:328–332
    [Google Scholar]
  15. Nasuno S., Starr M. P. 1966a; Polygalacturonase of Erwinia carotovora . Journal of Biological Chemistry 241:5298–5306
    [Google Scholar]
  16. Nasuno S., Starr M. P. 1966b; Pectic enzymes of Pseudomonas marginalis . Phytopathology 56:1414–1415
    [Google Scholar]
  17. Nasuno S., Starr M. P. 1967; Polygalacturonic acid transeliminase of Xanthomonas campestris. . Biochemical Journal 104:178–185
    [Google Scholar]
  18. Okamoto K., Hatanaka C., Ozawa J. 1965; The thiobarbituric acid test of 4,5-unsaturated digalacturonic acid. Berichte des Ohara Instituts für landwirtschaftliche Biologie 13:7–12
    [Google Scholar]
  19. Preiss J., Ashwell G. 1963; Polygalacturonic acid metabolism in bacteria. I. Enzymic formation of 4-deoxy-l-/Areo-5-hexosulose uronic acid. Journal of Biological Chemistry 238:1571–1576
    [Google Scholar]
  20. Rexova-Benkova L. 1970; Separation of oligo- galacturonic acids by dextran gel chromatography. Chemicky Zvesti 24:59–62
    [Google Scholar]
  21. Rombouts F. M. 1972; Occurrence and properties of bacterial pectate lyases. Agricultural Research Reports 779 Wageningen: Centre for Agricultural Publishing and Documentation;
    [Google Scholar]
  22. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  23. Starr M. P., Nasuno S. 1967; Pectolytic activity of phytopathogenic xanthomonads. Journal of General Microbiology 46:425–443
    [Google Scholar]
  24. Tomerska H. 1971; Decomposition of pectin in vitro by pure strains of rumen bacteria. Acta microbiologica polonica, Ser. B 3(20):107–115
    [Google Scholar]
  25. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature; London: 166444–445
    [Google Scholar]
  26. Ward O. P., Fogarty W. M. 1974; Polygalacturonate lyase production by Bacillus subtilis and Flavobacterium pectinovorum . Applied Microbiology 27:346–350
    [Google Scholar]
  27. Wojciechowicz M. 1971; Partial characterization of pectinolytic enzymes of Bacteroides ruminicola isolated from the rumen of a sheep. Acta microbiologica polonica, Ser. A 3(20):45–56
    [Google Scholar]
  28. Wojciechowicz M. 1972; Comparison of the action of Bacteroides ruminicola polygalacturonic acid lyase and of pectinase on lower oligogalacturonides. Acta microbiologica polonica, Ser. A 4(21):189–195
    [Google Scholar]
  29. Wojciechowicz M., Tomerska H. 1971; Pectic enzymes in some pectinolytic rumen bacteria. Acta microbiologica polonica, Ser. A 3(20):57–61
    [Google Scholar]
  30. Wojciechowicz M., Ziolecki A. 1979; Pectinolytic enzymes of large rumen treponemes. Applied and Environmental Microbiology 37:136–142
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-1-193
Loading
/content/journal/micro/10.1099/00221287-117-1-193
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error