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Abstract
Listeria specific antigen 2 (Ag2) was purified to within 97 % of homogeneity, with a high yield, using both gel filtration and polyacrylamide gel electrophoresis. Ag2 is a glycoprotein. Its isoelectric point is about 4·2. As determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, its molecular weight is 16710 ± 450. Ag2 may aggregate easily since it was previously found in gel filtration in a peak corresponding to a molecular weight of 160000. No enzyme activity has been found in Ag2.
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© Society for General Microbiology 1980