1887

Abstract

The addition of cholesterol to a liquid medium containing bovine serum albumin (BSA) fraction V or acetone-delipidized BSA fraction V instead of serum stimulated the growth of , a serum-requiring spirochaete associated with swine dysentery. As little as 1·25 g cholesterol ml increased viable counts about 1000-fold. Sitosterol and cholestanol, but not pregnenalone, cholestenone or stigmasterol, produced a growth response comparable to that of cholesterol. The results suggest that requires a sterol for growth.

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1980-02-01
2021-05-05
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References

  1. Edward D. G. ff., Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. Journal of General Microbiology 5:576–586
    [Google Scholar]
  2. Ellinghausen H. C. Jr McCullough W. G. 1965; Nutrition of Leptospira pomona and growth of 13 other serotypes: a serum free medium employing oleic acid albumin complex. American Journal of Veterinary Research 26:39–44
    [Google Scholar]
  3. Freundt E. A., Andrews B. E., Ernø M., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol-sulphonate and digitonin. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 225:104–112
    [Google Scholar]
  4. Harris D. L., Glock R. D., Christensen C. R., Kinyon J. M. 1972; Swine dysentery-I. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Veterinary Medicine/Small Animal Clinician 67:61–64
    [Google Scholar]
  5. Hovind-Hougen K. 1976; Determination by means of electron microscopy of morphological criteria of value for classification of some spirochetes, in particular treponemes. Actapathologica et microbiologica scandinavica B255: Supplement 1–41
    [Google Scholar]
  6. Johnson R. C., Eggebraten L. M. 1971; Fatty acid requirement of the Kazan 5 and Reiter strains of Treponema pallidum. Infection and Immunity 3:723–726
    [Google Scholar]
  7. Lemcke R. M., Bew J., Burrows M. R., Lysons R. J. 1979; The growth of Treponema hyodysenteriae and other porcine intestinal spirochaetes in a liquid medium. Research in Veterinary Science 26:315–319
    [Google Scholar]
  8. Meyer H., Meyer F. 1971; Lipid metabolism in the parasitic and free-living spirochaetes Treponema pallidum (Reiter) and Treponema zuelzerae. Biochimica et biophysica acta 231:93–106
    [Google Scholar]
  9. Oyama V. I., Steinman H. G., Eagle H. 1953; The nutritional requirements of treponemata. V. A detoxified lipid as the essential growth factor supplied by crystallized serum albumin. Journal of Bacteriology 65:609–616
    [Google Scholar]
  10. Rajkovic A. D. 1967; Cultivation of the Noguchi strain of Treponema pallidum in a lipid medium, in the absence of serum. Zeitschrift für medizinische Mikrobiologie und Immunologie 153:297–312
    [Google Scholar]
  11. Razin S. 1978; The mycoplasmas. Microbiological Reviews 42:414–470
    [Google Scholar]
  12. Razin S., Rottem S. 1963; Fatty acid requirements of Mycoplasma laidlawii. Journal of General Microbiology 33:459–470
    [Google Scholar]
  13. Razin S., Rottem S. 1978; Cholesterol in membranes: studies with mycoplasmas. Trends in Biochemical Sciences 3:51–55
    [Google Scholar]
  14. Rodwell A. W. 1963; The steroid growth-requirement of Mycoplasma mycoides. Journal of General Microbiology 32:91–101
    [Google Scholar]
  15. Smibert R. M. 1976a; Classification of nonpathogenic treponemes, borrelia and spirochaeta. In The Biology of Parasitic Spirochaetes pp. 121–131 Johnson R. C. Edited by New York: Academic Press;
    [Google Scholar]
  16. Smibert R. M. 1976b; Cultivation, composition and physiology of avirulent treponemes. In The Biology of Parasitic Spirochaetes pp. 49–56 Johnson R. C. Edited by New York: Academic Press;
    [Google Scholar]
  17. Smith P. F., Lynn R. J. 1958; Lipid requirements for the growth of pleuropneumonia-like organisms. Journal of Bacteriology 76:264–269
    [Google Scholar]
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