The Cell Surface in Amoeboid Locomotion: Behaviour of on an Adhesive Lectin Substrate Free

Abstract

The behaviour of amoebae on coverslips coated with concanavalin A was followed by reflection interference microscopy. Locomotion continued briefly on the protein and much material was shed before the cells halted. The effect was overcome by perfusion with hapten sugars. These carbohydrates dissociated those trails formed in water from the substrate save for areas of focal contact, whereas trails generated in 10 m-KCl were unaffected by hapten. These results argue for direct cell-substrate contact over a limited portion of the cell surface during this type of locomotion.

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/content/journal/micro/10.1099/00221287-116-2-515
1980-02-01
2024-03-29
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References

  1. Goldstein I. J., Hollerman C. E., Smith E. E. 1965; Protein-carbohydrate interaction. II. Inhibition studies on the interaction of con- canavalin A with polysaccharides. Biochemistry 4:876–883
    [Google Scholar]
  2. Heaysman J. E. M. 1978; Contact inhibition of locomotion: a reappraisal. International Review of Cytology 55:49–65
    [Google Scholar]
  3. King C. A., Westwood R., Cooper L., Preston T. M. 1979; Speed of locomotion of the soil amoeba Naegleria gruberi in media of different ionic compositions with special reference to interactions with the substratum. Protoplasma 99:323–334
    [Google Scholar]
  4. Lis H., Sharon N. 1973; The biochemistry of plant lectins (phytohemagglutinins). Annual Review of Biochemistry 42:541–574
    [Google Scholar]
  5. O’Dell D. S., Preston T. M., King C. A., Gardiner P. R. 1976; An approach to cell surface receptors in an amoebo-flagellate protozoon. Biochemical Society Transactions 4:124–125
    [Google Scholar]
  6. Preston T. M., King C. A. 1978a; Cell substrate associations during the amoeboid locomotion of Naegleria. Journal of General Microbiology 104:347–351
    [Google Scholar]
  7. Preston T. M., King C. A. 1978b; A.n experimental study of the interactions between the soil amoeba Naegleria gruberi and a glass substrate during amoeboid locomotion. Journal of Cell Science 34:145–158
    [Google Scholar]
  8. Preston T. M., O’Dell D. S., King C. A. 1975; Fluorescence microscope observations of some surface components of the amoebo- flagellate Naegleria gruberi during amoeboid locomotion. Cytobios 13:207–216
    [Google Scholar]
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