1887

Microbiology Society logo

Volume 116, Issue 2

Studies of Temperature-sensitive Transfer and Maintenance of H Incompatibility Group Plasmids Free

Abstract

The mechanism of temperature-sensitive transfer was studied for plasmids of the H incompatibility group. Transfer depended on the temperature of the mating mixture but the growth temperature of the donor was also important, and donor cells previously grown at 26 °C could not facilitate transfer at 37°C. Comparison of transfer characteristics of a non-thermosensitive H plasmid R831b and thermosensitive H plasmids from from Ontario during a 2 h mating period showed that the thermosensitive phenotype inhibited transfer by about 200-fold at 37 °C and by 10-fold at 42 °C. At temperatures between 15 and 30 °C, the thermotolerant H plasmid transferred at about the same frequency as the temperature-sensitive plasmid. Elimination of some H plasmids after growth of host cells was also observed and physical evidence of this was obtained. The characteristic of high-temperature elimination (Hte) was limited to plasmids from similar bacterial and geographical sources. Plasmids from spp. isolated in Ontario did not possess this phenotype, whereas plasmids from isolated in the United States did. Although the Tra(ts) and Hte phenotypes may both be characteristic of H plasmids, they were shown to be separate and distinct properties. The H plasmids used in this study were isolated and their molecular weights determined by agarse gel electrophoresis. All were large, with molecular weights often exceeding 140 × 10. In contrast, the thermostable H plasmid R831b had a molecular weight of only 49 × 10.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1980
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-2-475
1980-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/2/mic-116-2-475.html?itemId=/content/journal/micro/10.1099/00221287-116-2-475&mimeType=html&fmt=ahah

References

  1. Achtman M., Skurray R. 1977; A redefinition of the mating phenomenon in bacteria. In Microbial Interactions (Receptors and Recognition Series B, vol. 3 pp. 234–279 Edited by Reissig J. L. London: Chapman and Hall;
     [Google Scholar]
  2. Anderson E. S. 1975; The problem and implications of chloramphenicol resistance in the typhoid bacillus. Journal of Hygiene 74:289–299
     [Google Scholar]
  3. Anderson E. S. 1977; The geographical predominance of resistance transfer systems of various compatibility groups in Salmonellae. In R-factors: Their Properties and Possible Control Topics in Infectious Disease, vol. 2 pp. 25–38 Edited by Drews J., Hogenauer G. Wien: Springer-Verlag;
     [Google Scholar]
  4. Anderson E. S., Humphreys G. O., Willshaw G. A. 1975; The molecular relatedness of R factors in enterobacteria of human and animal origin. Journal of General Microbiology 91:376–382
     [Google Scholar]
  5. Barth P. T., Richards H., Datta N. 1978; Copy number of coexisting plasmids in Escherichia coli K12. Journal of Bacteriology 135:760–765
     [Google Scholar]
  6. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri . Journal of General Microbiology 72:543–552
     [Google Scholar]
  7. Dijoseph C. G., Kaji A. 1974; The thermosensitive lesion in the replication of the drug resistance factor, Rtsl. Proceedings of the National Academy of Sciences of the United States of America 712515–2519
     [Google Scholar]
  8. Grant R. B., Di Mambro L. 1977; Antimicrobial resistance and resistance plasmids in Salmonella from Ontario, Canada. Canadian Journal of Microbiology 23:1266–1273
     [Google Scholar]
  9. Grant R. B., Bannatyne R. M., Shapley A. 1976; Chloramphenicol and ampicillin resistant Salmonella typhimurium in Ontario. Journal of Infectious Diseases 134:354–361
     [Google Scholar]
  10. Grindley N. D. F., Grindley J. N., Anderson E. S. 1972; R factor compatibility groups. Molecular and General Genetics 119:287–297
     [Google Scholar]
  11. Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Molecular studies of R factor compatibility groups. Journal of Bacteriology 115:387–398
     [Google Scholar]
  12. Hansen J. B., Olsen R. H. 1978a; Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMGl and pMG5. Journal of Bacteriology 135:227–238
     [Google Scholar]
  13. Hansen J. B., Olsen R. H. 1978b; IncP2 group of Pseudomonas, a class of uniquely large plasmids. Nature, London 274:715–717
     [Google Scholar]
  14. Hedges R. W., Rodriguez-Lemoine V., Datta N. 1975; R factors from Serratia marcescens . Journal of General Microbiology 86:88–92
     [Google Scholar]
  15. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids, and Episomes pp. 607–638 Edited by Bukhari A. I., Shapiro J. A., Adhya S. L. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  16. Johnson E. M., Wohlhieter J. A., Placek B. P., Sleet R. B., Baron L. S. 1976; Plasmid- determined ability of a Salmonella tennessee strain to ferment lactose and sucrose. Journal of Bacteriology 125:385–386
     [Google Scholar]
  17. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1537
     [Google Scholar]
  18. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug- resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:55–83
     [Google Scholar]
  19. Novick R. P., Clowes R. C., Cohen S. N., Curtissiii R., Datta N., Falkow S. 1976; Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40:168–189
     [Google Scholar]
  20. Rodriguez-Lemoine V., Jacob A. E., Hedges R. W., Datta N. 1975; Thermosensitive production of their transfer system by group S plasmids. Journal of General Microbiology 86:111–114
     [Google Scholar]
  21. Romero E., Perduca M. 1977; Compatibility groups of R-factors for trimethoprim resistance isolated in Italy. Journal of Antimicrobial Chemotherapy 3: suppl. C 35–38
     [Google Scholar]
  22. Roussel A. F., Chabbert Y. A. 1978; Taxonomy and epidemiology of Gram-negative bacterial plasmids studied by DNA-DNA filter hybridization in formamide. Journal of General Microbiology 104:269–276
     [Google Scholar]
  23. Smith H. W. 1974; Thermosensitive transfer factors in chloramphenicol-resistant strains of Salmonella typhi . Lancet ii:281
     [Google Scholar]
  24. Smith H. W., Parsell Z., Green P. 1978; Thermosensitive antibiotic resistance plasmids in enterobacteria. Journal of General Microbiology 109:37–47
     [Google Scholar]
  25. Taylor D. E., Grant R. B. 1976; Inhibition of bacteriophage lambda, Tl, and T7 development by R plasmids of the H incompatibility group. Antimicrobial Agents and Chemotherapy 10:762–764
     [Google Scholar]
  26. Taylor D. E., Grant R. B. 1977a; Incompatibility and bacteriophage inhibition properties of N-l, a plasmid belonging to the H2 incompatibility group. Molecular and General Genetics 153:5–10
     [Google Scholar]
  27. Taylor D. E., Grant R. B. 1977b; R plasmids of the S incompatibility group belong to the H2 incompatibility group. Antimicrobial Agents and Chemotherapy 12:431–434
     [Google Scholar]
  28. Taylor D. E., Grant R. B. 1978; Bacteriophage inhibition properties of H2 plasmids. In Microbiology -1978 pp. 214–216 Edited by Schlessinger D. Washington: American Society for Microbiology;
     [Google Scholar]
  29. Taylor D. E., Summers A. O. 1979; Association of tellurium resistance and bacteriophage inhibition conferred by R plasmids. Journal of Bacteriology 137:1430–1433
     [Google Scholar]
  30. Taylor D. E., Shermer M., Grant R. B. 1978; Incidence of the H2 group of plasmids in chloramphenicol-sensitive salmonella isolated in 1974 from clinical sources in Ontario. Canadian Journal of Microbiology 24:600–607
     [Google Scholar]
  31. Terawaki Y., Takayasu H., Akiba T. 1967; Thermosensitive replication of a kanamycin resistance factor. Journal of Bacteriology 94:687–690
     [Google Scholar]
  32. Watanabe T., Fukasawa T. 1961; Episome- mediated transfer of drug resistance in Entero- bacteriaceae. I. Transfer of resistance factors by conjugation. Journal of Bacteriology 81:669–678
     [Google Scholar]
  33. Watanabe T., Nishida H., Ogata C., Arai T., Sato S. 1964; Episome mediated transfer of drug-resistance in Enterobacteriaceae. VII. Two types of naturally occurring R factors. Journal of Bacteriology 88:716–726
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-2-475
Loading
Studies of Temperature-sensitive Transfer and Maintenance of H Incompatibility Group Plasmids
Microbiology 116, 475 (1980); https://doi.org/10.1099/00221287-116-2-475
/content/journal/micro/10.1099/00221287-116-2-475
/content/journal/micro/10.1099/00221287-116-2-475
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error