1887

Abstract

The chemical nature of the antigens of the meningococcal serotypes described by Frasch and colleagues was determined by a combination of immunodiffusion and the SDS-poly-acrylamide gel electrophoresis immunoperoxidase technique (SGIP). It was confirmed that the serotype antigens of the outer membrane of serotypes 1, 2, 6, 9, 11 and 12 were proteins, whilst those of serotypes 4, 5 and 8 were lipopolysaccharides. Serotype 2 can now be divided into three related types, provisionally called 2a (originally serotype 2), 2b and 2c with the specific antigens being proteins having molecular weights of 41000, 41500 and 41500, respectively. A total of 195 strains of meningococci isolated from patients and carriers in the Netherlands and 20 serogroup Y strains from patients in the U.S.A. were serotyped by means of immunodiffusion. Serotype 2a could be demonstrated in some strains belonging to the serogroups B (only those from carriers), C, W-135 and Y (only those from the U.S.A.). The W-135 strains isolated from patients in this series more often belonged to serotype 2a than did the W-135 strains from carriers. Serotype 2b was present in about half of the serogroup B and a few serogroup C strains isolated from patients with meningitis, but absent in serogroup B and C strains from carriers. Serotype 2c could only be demonstrated in serogroup Y strains, both from the Netherlands and the U.S.A. The other serotypes were found only sporadically.

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1980-02-01
2024-12-08
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References

  1. Bhattacharjee A. K., Jennings H. J., Kenny C. P. 1978; Structural elucidation of the 3- deoxy-D-wawio-octulosonic acid containing meningococcal 29-e capsular polysaccharide antigen using car bon-13 nuclear magnetic resonance. Biochemistry 17:645–651
    [Google Scholar]
  2. Branham S. E. 1953; Serological relationships among meningococci. Bacteriological Reviews 17:175–188
    [Google Scholar]
  3. COMMUNICABLE DISEASE SURVEILLANCE CENTRE 1978; Acute meningococcal infections; 1977 British Medical Journal 11560
    [Google Scholar]
  4. Evans J. R., Artenstein M. S., Hunter D. H. 1968; Prevalence of meningococcal serogroups and description of three new groups. American Journal of Epidemiology 87:643–646
    [Google Scholar]
  5. Frasch C. E. 1977; Role of protein serotype antigens in protection against disease due to Neisseria meningitidis. . Journal of Infectious Diseases 136S:S84–S90
    [Google Scholar]
  6. Frasch C. E., Chapman S. S. 1972a; Classification of Neisseria meningitidis group B into distinct serotypes. I. Serological typing by a microbactericidal method. Infection and Immunity 5:98–102
    [Google Scholar]
  7. Frasch C. E., Chapman S. S. 1972b; Classification of Neisseria meningitidis group B into distinct serotypes. II. Extraction of type-specific antigens for serotyping by precipitin techniques. Infection and Immunity 6:127–133
    [Google Scholar]
  8. Frasch C. E., Chapman S. S. 1973; Classification of Neisseria meningitidis into distinct serotypes. III. Application of a new bactericidal- inhibition technique to distribution of serotypes among cases and carriers. Journal of Infectious Diseases 127:149–154
    [Google Scholar]
  9. Frasch C. E., Friedman G. L. 1977; Identification d’un serotype meningococcique associe a la maladie et commun aux meningocoques des serogroupes B, C, Y et 135 W. Médecine tropicale 37:155–159
    [Google Scholar]
  10. Frasch C. E., Gotschlich E. C. 1974; An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. Journal of Experimental Medicine 140:87–104
    [Google Scholar]
  11. Frasch C. E., Mcnelis R. M., Gotschlich E. C. 1976; Strain-specific variation in the protein and lipopolysaccharide composition of the group B meningococcal outer membrane. Journal of Bacteriology 127:973–981
    [Google Scholar]
  12. Gold R., Wyle F. A. 1970; New classification of Neisseria meningitidis by means of bactericidal reactions. Infection and Immunity 1:479–484
    [Google Scholar]
  13. Gold R., Winkelhake J. L., Mars R. S., Artenstein M. S. 1971; Identification of an epidemic strain of group C Neisseria meningitidis by bactericidal serotyping. Journal of Infectious Diseases 124:593–597
    [Google Scholar]
  14. Jennings H. J., Bhattacharjee A. K., Bundle D. R., Kenny C. P., Martin A., Smith I. C. P. 1977; Structures of the capsular polysaccharides of Neisseria meningitidis as determined by 13C-nuc!ear magnetic resonance spectroscopy. Journal of Infectious Diseases 136S:S71–S77
    [Google Scholar]
  15. Jones D. M., Tobin B. M. 1976; Serotypes of group B meningococci. Journal of Clinical Pathology 29:746–748
    [Google Scholar]
  16. Mandrell R. E., Zollinger W. D. 1977; Lipopolysaccharide serotyping of Neisseria meningitidis by hemagglutination inhibition. Infection and Immunity 16:471–475
    [Google Scholar]
  17. MENINGOCOCCADLIS EASES URVEILLANCE GROUP 1976; Analysis of endemic meningococcal disease by serogroup and evaluation of chemoprophylaxis. Journal of Infectious Diseases 134201–204
    [Google Scholar]
  18. Munford R. S., Patton C. M., Gorman G. W. 1975; Epidemiological studies of serotype antigens common to groups B and C Neisseria meningitidis . Journal of Infectious Diseases 131:286–290
    [Google Scholar]
  19. Poolman J. T., Hopman C. T. P., Zanen H. C. 1978; Immunochemical characterization of outer membrane complexes from Neisseria meningitidis by the SDS-polyacrylamide-gel-electro- phoresis-immuno-peroxidase technique (SGIP). FEMS Microbiology Letters 4:245–248
    [Google Scholar]
  20. Raamsdonk W. v., Pool C. W., Heyting C. 1977; Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels. Journal of Immunological Methods 17:337–348
    [Google Scholar]
  21. Slaterus K. W. 1961; Serological typing of meningococci by means of micro-precipitation. Antonie van Leeuwenhoek 27:305–315
    [Google Scholar]
  22. Zollinger W. D., Mandrell R. E. 1977; Outer-membrane protein and lipopolysaccharide serotyping of Neisseria meningitidis by inhibition of a solid-phase radioimmunoassay. Infection and Immunity 18:424–433
    [Google Scholar]
  23. Zollinger W. D., Kasper D. L., Veltri B. J., Artenstein M. S. 1972; Isolation and characterization of a native cell wall complex from Neisseria meningitidis. . Infection and Immunity 6:835–851
    [Google Scholar]
  24. Zollinger W. D., Pennington C. L., Artenstein M. S. 1974; Human antibody response to three meningococcal outer membrane antigens: comparison by specific hemagglutination assays. Infection and Immunity 10:975–984
    [Google Scholar]
  25. Zollinger W. D., Mandrell R. E., Altieri P., Berman S., Lowenthal J., Artenstein M. S. 1978; . Safety and immunogenicity of a Neisseria meningitidis type 2 protein vaccine in animals and humans. Journal of Infectious Diseases 137:728–739
    [Google Scholar]
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