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The interactions of bacteria, mainly Serratia marinorubra and Pseudomonas halocrenaea, with different lipid surface films were examined employing the surface balance technique and particle electrophoresis. Three free fatty acids as well as octadecylamine, and two diacyl-phosphatidylcholines were used as monolayer films with phosphate and ammonium acetate buffers as subsurface solutions. The following factors were involved in the interaction: the architecture of the surface film, the charge carried by both the bacteria and the film, hydrophobic interaction, and probably enzyme activity. The presence of Ca2+ seemed to be necessary for interfacial phospholipase activity.
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