1887

Abstract

Glucose-limited cultures of NCTC 418 (and the supposedly identical strain NCIB 418) possessed a glucose phosphopyruvate (PEP) phosphotransferase activity that varied markedly and progressively with growth rate, from more than 250 nmol min (mg dry wt cells) at = 0·1 h to less than 100 nmol min (mg dry wt cells) at = 0·8 h. When relieved of the glucose limitation, substrate was used at a rate that bore no precise relationship to the cells’ phosphotransferase activity. Similarly, glucose-sufficient (phosphate- or potassium-limited) cultures metabolized glucose at high rates, whereas the cells possessed only moderate glucose PEP phosphotransferase activities. These results are compared with those reported for glucose-limited cultures of and for variously limited cultures of Glucose-sufficient cultures, as well as glucose-limited cultures that had been temporarily relieved of glucose limitation, excreted partially oxidized products of glucose catabolism in considerable amounts. The relevance of this ‘overflow’ metabolism to studies of glucose transport using [U-C]glucose is emphasized.

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1980-02-01
2024-03-29
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References

  1. Carter I. S., Dean A. C. R. 1977; Hexokinase and glucose-phosphoenolpyruvate phosphotransferase synthesis in Klebsiella aerogenes strains growing in continuous culture. Biochemical Journal 166:643–646
    [Google Scholar]
  2. Cooper C. M., Fernstrom G. A., Miller S. A. 1944; Performance of agitated gas-liquid contactors. Industrial and Engineering Chemistry 36:504–509
    [Google Scholar]
  3. Curtis S. J., Epstein W. 1975; Phosphorylation of d-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. Journal of Bacteriology 122:1189–1199
    [Google Scholar]
  4. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous cultivation of microorganisms, II.Construction of a chemostat. Methods in Microbiology 2:275–327
    [Google Scholar]
  5. Harvey R. J. 1970; Metabolic regulation in glucose-limited chemostat cultures of Escherichia coli . Journal of Bacteriology 104:698–706
    [Google Scholar]
  6. Herbert D. 1961; The chemical composition of microorganisms as a function of their environment. Symposia of the Society for General Microbiology 11:391–416
    [Google Scholar]
  7. Herbert D. 1976; Stoicheiometric aspects of microbial growth. In Continuous Culture 6. Applications and New Fields pp. 1–30 Dean A. C. R., Ellwood D. C., Evans C. G. T., Melling j. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  8. Herbert D., Kornberg H. L. 1976; Glucose transport as rate-limiting step in the growth of Escherichia coli on glucose. Biochemical Journal 156:477–480
    [Google Scholar]
  9. Herbert D., Phipps P. J., Tempest D. W. 1965; The chemostat: design and instrumentation. Laboratory Practice 14:1150–1161
    [Google Scholar]
  10. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  11. Hunter I. S., Kornberg H. L. 1979; Glucose transport of Escherichia coli in glucose-limited continuous culture. Biochemical Journal 178:97–101
    [Google Scholar]
  12. Kornberg H. L. 1976; Genetics in the study of carbohydrate transport by bacteria. Journal of General Microbiology 96:1–16
    [Google Scholar]
  13. Kornberg H. L., Reeves R. E. 1972a; Correlation between hexose transport and phosphotransferase activity in Escherichia coli . Biochemical Journal 126:1241–1243
    [Google Scholar]
  14. Kornberg H. L., Reeves R. E. 1972b; Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coli . Biochemical Journal 128:1339–1344
    [Google Scholar]
  15. Kundig W., Ghosh S., Roseman S. 1964; Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system. Proceedings of the National Academy of Sciences of the United States of America 52:1067–1074
    [Google Scholar]
  16. Lin E. C. C. 1970; The genetics of bacterial transport systems. Annual Review of Genetics 4:225–262
    [Google Scholar]
  17. Monod J. 1942 Recherches sur la Croissance des Cultures Bácteriennes Paris: Hermann et Cie;
    [Google Scholar]
  18. Neijssel O. M., Tempest D. W. 1975a; Production of gluconic acid and 2-ketogluconic acid by Klebsiella aerogenes NCTC418. Archives of Microbiology 105:183–185
    [Google Scholar]
  19. Neijssel O. M., Tempest D. W. 1975b; The regulation of carbohydrate metabolism in Klebsiella aerogenes NCTC 418 organisms, growing in chemostat culture. Archives of Microbiology 106:251–258
    [Google Scholar]
  20. Neijssel O. M., Tempest D. W. 1976; The role of energy-spilling reactions in the growth of Klebsiella aerogenes NCTC 418 in aerobic chemostat culture. Archives of Microbiology 110:305–311
    [Google Scholar]
  21. Neijssel O. M., Hueting S., Tempest D. W. 1977; Glucose transport capacity is not the rate-limiting step in the growth of some wild-type strains of Escherichia coli and Klebsiella aerogenes in chemostat culture. FEMS Microbiology Letters 2:1–3
    [Google Scholar]
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