1887

Abstract

has at least two permeases for -proline. The gene of the gene cluster specifies the major proline permease, which is inducible by proline. Synthesis of the permease is subject to repression by ammonium at 37 °C but not at 25 °C. A genetically unidentified minor proline permease(s) does not respond to proline induction or ammonium repression but is inhibited by ammonium. mutants are unable to utilize nitrogen sources other than ammonium because they lack a positive-acting regulatory product required for expression of ammonium-repressible activities. However, there are very few cases in which the lack of growth of mutants on a particular nitrogen source can be attributed to a reduction in the level of a particular enzyme activity or permease. Reduced expression of the permease can account for the inability of mutants to utilize proline. This is demonstrated by the ability of -acting regulatory mutations designated , which derepress synthesis of the permease, to suppress mutations for proline utilization. The apparent ability of mutations to derepress synthesis of proline oxidase and l-pyrroline-5-carboxylate dehydrogenase is probably an indirect consequence of their ability to derepress synthesis of the permease, preventing inducer exclusion. There is presently no evidence that mutations directly affect expression of the , or genes, but this possibility has not been definitively eliminated.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-2-285
1980-02-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/2/mic-116-2-285.html?itemId=/content/journal/micro/10.1099/00221287-116-2-285&mimeType=html&fmt=ahah

References

  1. Arst H. N. Jr Bailey C. R. 1977; The regulation of carbon metabolism in Aspergillus nidulans . In Genetics and Physiology of Aspergillus pp. 131–146 Smith J. E., Pateman J. A. Edited by London: Academic Press;
    [Google Scholar]
  2. Arst H. N. Jr Cove D. J. 1969; Methyl- ammonium resistance in Aspergillus nidulans . Journal of Bacteriology 98:1284–1293
    [Google Scholar]
  3. Arst H. N. Jr Cove D. J. 1973; Nitrogen metabolite repression in Aspergillus nidulans . Molecular and General Genetics 126:111–141
    [Google Scholar]
  4. Arst H. N. Jr Macdonald D. W. 1975; A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region. Nature; London: 25426–31
    [Google Scholar]
  5. Arst H. N. Jr Macdonald D. W. 1978; Reduced expression of a distal gene of the prn gene cluster in deletion mutants of Aspergillus nidulans: genetic evidence for a dicistronic messenger in an eukaryote. Molecular and General Genetics 163:17–22
    [Google Scholar]
  6. Arst H. N. Jr Page M. M. 1973; Mutants of Aspergillus nidulans altered in the transport of methylammonium and ammonium. Molecular and General Genetics 121:239–245
    [Google Scholar]
  7. Arst H. N. Jr Scazzocchio C. 1975; Initiator constitutive mutation with an ‘up-promoter’ effect in Aspergillus nidulans . Nature; London: 25431–34
    [Google Scholar]
  8. Arst H. N. Jr Macdonald D. W., Jones S. A. 1977; The prn gene cluster in Aspergillus nidulans . Heredity 39:434
    [Google Scholar]
  9. Arst H. N. Jr Rand K. N., Bailey C. R. 1979; Do the tightly linked structural genes for nitrate and nitrite reductases in Aspergillus nidulans form an operon? Evidence from an insertional translocation which separates them. Molecular and General Genetics 174:89–100
    [Google Scholar]
  10. Bailey C., Arst H. N. Jr 1975; Carbon catabolite repression in Aspergillus nidulans . European Journal of Biochemistry 51:573–577
    [Google Scholar]
  11. Bailey C. R., Penfold H. A., Arst H. N. Jr 1979; CA-dominant regulatory mutations affecting the expression of GABA permease in Aspergillus nidulans. . Molecular and General Genetics 169:79–83
    [Google Scholar]
  12. Clutterbuck A. J. 1974; Aspergillus nidulans. In Handbook of Genetics 1 pp. 447–510 King R. C. Edited by New York: Plenum Press;
    [Google Scholar]
  13. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. . Biochimica et biophysica acta 113:51–56
    [Google Scholar]
  14. Cove D. J. 1976; Chlorate toxicity in Aspergillus nidulans. Studies of mutants altered in nitrate assimilation. Molecular and General Genetics 146:147–159
    [Google Scholar]
  15. Drainas C., Kinghorn J. R., Pateman J. A. 1977; Aspartic hydroxamate resistance and asparaginase regulation in the fungus Aspergillus nidulans . Journal of General Microbiology 98:493–501
    [Google Scholar]
  16. Dunn E., Pateman J. A. 1972; Urea and thiourea uptake in Aspergillus nidulans. . Heredity 29:129
    [Google Scholar]
  17. Facklam T. J., Marzluf G. A. 1978; Nitrogen regulation of amino acid catabolism in Neurospora crassa . Biochemical Genetics 16:343–354
    [Google Scholar]
  18. Hynes M. J. 1975a; Studies on the role of the areA gene in the regulation of nitrogen catabolism in Aspergillus nidulans . Australian Journal of Biological Sciences 28:301–313
    [Google Scholar]
  19. Hynes M. J. 1975b; A cA-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans . Nature; London : 253210–212
    [Google Scholar]
  20. Käfer E. 1962; Translocations in stock strains of Aspergillus nidulans . Genetica 33:59–68
    [Google Scholar]
  21. Layne E. 1957; Spectrophotometric and turbidi- metric methods for measuring proteins. Methods in Enzymology 3:447–454
    [Google Scholar]
  22. Mccully K. S., Forbes E. 1965; The use of P-fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6:352–359
    [Google Scholar]
  23. Pontecorvo G., Roper J. A., Hemmons L. A., Macdonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans . Advances in Genetics 5:141–238
    [Google Scholar]
  24. Rand K. N. Aspects of the control of nitrogen metabolism in Aspergillus nidulans Ph.D. thesis University of Cambridge:
    [Google Scholar]
  25. Rand K. N., Arst H. N. Jr 1977; A mutation in Aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene. Molecular and General Genetics 155:67–75
    [Google Scholar]
  26. Rand K. N., Arst H. N. Jr 1978; Mutations in nirA gene of Aspergillus nidulans and nitrogen metabolism. Nature; London: 272732–734
    [Google Scholar]
  27. Williams I., Frank L. 1975; Improved chemical synthesis and enzymatic assay of △1-pyrroline-5- carboxylic acid. Analytical Biochemistry 64:85–97
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-2-285
Loading
/content/journal/micro/10.1099/00221287-116-2-285
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error