1887

Abstract

Transfer of derivatives of the bacteriocinogenic plasmid pRL1JI into eight symbiotically defective strains of resulted in suppression of the mutant phenotype in four cases. These included non-infective, ineffective and temperature-sensitive ineffective phenotypes. However, in none of these strains was the defect suppressible after high frequency transfer of derivatives of two other bacteriocinogenic plasmids, pRL3JI or pRL4JI. Nodulation ability was co-transferred at high frequency (> 95 %) with bacteriocin production by the plasmid pRL1JI. The other two plasmids, pRL3JI and pRL4JI, also mediated the transfer of nodulation ability but at much lower frequency (10 to 10 per plasmid transfer). Sometimes, transconjugants that had acquired nodulation ability after the transfer of derivatives of plasmids pRL3JI or pRL4JI acted subsequently as high frequency donors for nodulation ability in a manner that was apparently similar to strains containing pRL1JI.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-2-261
1980-02-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/2/mic-116-2-261.html?itemId=/content/journal/micro/10.1099/00221287-116-2-261&mimeType=html&fmt=ahah

References

  1. Berg D. E., Davies J., Allet B., Rochaix J. D. 1975; Transposition of R factor genes to bacteriophage λ. Proceedings of the National Academy of Sciences of the United States of America 72:3628–3632
    [Google Scholar]
  2. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum . Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beringer J. E., Hopwood D. A. 1976; Chromosomal recombination and mapping in Rhizobium leguminosarum . Nature, London 264:291–293
    [Google Scholar]
  4. Beringer J. E., Johnston A. W. B., Wells B. 1977; The isolation of conditional ineffective mutants of Rhizobium leguminosarum . Journal of General Microbiology 98:339–343
    [Google Scholar]
  5. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978a; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  6. Beringer J. E., Beynon J. L., Buchanan-Wollaston A. V., Johnston A. W. B. 1978b; Transfer of the drug-resistance transposon Tn5 to Rhizobium . Nature, London 276:633–634
    [Google Scholar]
  7. Buchanan-Wollaston V. 1979; Generalized transduction in Rhizobium leguminosarum . Journal of General Microbiology 112:135–142
    [Google Scholar]
  8. Casse F., Boucher C., Julliot J. S., Michel M., Dénarié J. 1979; Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. Journal of General Microbiology 113:229–242
    [Google Scholar]
  9. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa . Molecular and General Genetics 144:243–251
    [Google Scholar]
  10. Higashi S. 1967; Transfer of clover infectivity of Rhizobium trifolii to Rhizobium phaseoli as mediated by an episomic factor. Journal of General and Applied Microbiology 13:391–403
    [Google Scholar]
  11. Hirsch P. R. 1979; Plasmid-determined bacterio- cin production by Rhizobium leguminosarum . Journal of General Microbiology 113:219–228
    [Google Scholar]
  12. Johnston A. W. B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  13. Johnston A. W. B., Beringer J. E. 1976; Pea root nodules containing more than one Rhizobium species. Nature, London 263:502–504
    [Google Scholar]
  14. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium . Nature, London 276:635–636
    [Google Scholar]
  15. Johnston A. W. B., Beringer J. E., Beynon J. L., Brewin N. J., Buchanan-Wollaston A. V., Hirsch P. R. 1979; Involvement of plasmids in nodulation by Rhizobium . In Plasmids of Medical, Environmental and Commercial Importance pp. 317–325 Amsterdam: Elsevier/ North Holland;
    [Google Scholar]
  16. Josey D. P., Beynon J. L., Johnston A. W. B., Beringer J. E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  17. Nuti M. P., Ledeboer A. M., Lepidi A. A., Schilperoort R. A. 1977; Large plasmids in different Rhizobium species. Journal of General Microbiology 100:241–248
    [Google Scholar]
  18. Pain A. N. 1979; Symbiotic properties of antibiotic-resistant and auxotrophic mutants of Rhizobium leguminosarum . Journal of Applied Bacteriology 47:53–64
    [Google Scholar]
  19. Sutton W. D. 1974; Some features of the DNA of Rhizobiumbacteroids and bacteria. Biochimica et biophysica acta 336:1–10
    [Google Scholar]
  20. Tshitinge G., Luyindula N., Lurquin P. F., Ledoux L. 1975; Plasmid DNA in Rhizobium vigna and Rhizobium trifolii . Biochimica et biophysica acta 414:357–361
    [Google Scholar]
  21. Vincent J. M. 1970 A Manual for the Practical Study of the Root Nodule Bacteria IPB Handbook no. 15. Oxford & Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  22. Zurkowski W., Lorkiewicz Z. 1976; Plasmid deoxyribonucleic acid in Rhizobium trifolii . Journal of Bacteriology 128:481–484
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-2-261
Loading
/content/journal/micro/10.1099/00221287-116-2-261
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error