1887

Microbiology Society logo

Volume 116, Issue 1

Effects of Trialkyllead Compounds on Growth, Respiration and Ion Transport in K12 Free

Abstract

Triethyllead and tripropyllead cations affected growth, energy metabolism and ion transport in K12. The tripropyllead compound was more liposoluble than the triethyl analogue and was also more effective in inhibiting cell growth and the oxygen uptake of both intact cells and membrane particles. Triethyllead acetate (5 ) inhibited growth on non-fermentable carbon sources, such as glycerol and succinate, more markedly than on glucose. At higher concentrations, triethyllead caused significant inhibition of respiration rates of intact cells; the concentration giving 50 % inhibition was 60 for glycerol-grown cells and 158 for glucose-grown cells. Oxidation of succinate by membrane particles was less sensitive to inhibition by the tripropyl- or triethyllead compounds than were the oxidations of -lactate or NADH. Triethyllead acetate [1·9 mol (mg membrane protein)] inhibited the reduction by NADH of cytochromes; evidence for more than one site of inhibition in the respiratory chain was obtained. Membrane-bound ATPase activity was strongly inhibited by triethyllead acetate in the absence or presence of Cl. The concentration of inhibitor giving 50 % inhibition [0·02 mol (mg membrane protein)] was about two orders of magnitude lower than that required for 50 % inhibition of substrate oxidation rates in membranes. Triethyllead acetate (1 ) induced swelling of spheroplasts in iso-osmotic solutions of either NHCl or NHBr, presumably as a result of the mediation by the organolead compound of Cl/OH and Br/OH antiports across the cytoplasmic membrane. Similar exchanges of OH for F, NO or SO or the uniport of H could not be demonstrated. Comparisons are drawn between the effects of trialkyllead compounds and those of the more widely studied trialkyltin compounds.

  • Received:
  • Published Online:
© Society for General Microbiology 1980
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-1-99
1980-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/1/mic-116-1-99.html?itemId=/content/journal/micro/10.1099/00221287-116-1-99&mimeType=html&fmt=ahah

References

  1. Aldridge W. N. 1976; The influence of organotin compounds on mitochondrial functions. In Organotin Compounds: New Chemistry and Applications Advances in Chemistry Series 157 pp. 186–196 Zuckerman J. J. Edited by Washington: American Chemical Society;
     [Google Scholar]
  2. Aldridge W. N., Street B. W. 1964; Oxidative phosphorylation. Biochemical effects and properties of trialkyltins. Biochemical Journal 91:287–296
     [Google Scholar]
  3. Aldridge W. N., Street B. W. 1971; Oxidative phosphorylation: relation between specific binding of trimethyltin and triethyltin to mitochondria and their effects on various mitochondrial functions. Biochemical Journal 124:221–234
     [Google Scholar]
  4. Aldridge W. N., Street B. W., Skilleter D. N. 1977; Oxidative phosphorylation. Halide-dependent and halide-independent effects of tri-organotin and triorganolead compounds on mitochondrial functions. Biochemical Journal 168:353–364
     [Google Scholar]
  5. Bolanowska W. 1968; Distribution and excretion of triethyllead in rats. British Journal of Industrial Medicine 25:203–208
     [Google Scholar]
  6. Cain K., Partis M. D., Griffiths D. E. 1977a; Dibutylchloromethyltin chloride, a covalent inhibitor of the adenosine triphosphate synthase complex. Biochemical Journal 166:593–602
     [Google Scholar]
  7. Cain K., Hyams R. L., Griffiths D. E. 1977b; Studies on energy-linked reactions: inhibition of oxidative phosphorylation and energy-linked reactions by dibutyltin dichloride. FEBS Letters 82:23–28
     [Google Scholar]
  8. Chance B., Schoener B. 1966; High and low energy states of cytochromes. I. In mitochondria. Journal of Biological Chemistry 241:4567–4573
     [Google Scholar]
  9. Coleman J. O. D., Palmer J. M. 1971; The influence of pH on the inhibition of oxidative phosphorylation and electron transport by triethyltin. Biochimica et biophysica acta 245:313–320
     [Google Scholar]
  10. Cremer J. E. 1962; Tetraethyllead toxicity in rats. Nature; London: 195607–608
     [Google Scholar]
  11. Daniel R. M. 1970; The electron transport system of Acetobacter suboxydans with particular reference to cytochrome o. Biochimica et biophysica acta 216:328–341
     [Google Scholar]
  12. Dawson A. P., Selwyn M. J. 1974; The action of trialkyltin compounds on mitochondrial respiration. The effect of pH. Biochemical Journal 138:349–357
     [Google Scholar]
  13. Degn H., Lilleør M., Iversen J. J. L. 1973; The occurrence of a stepwise-decreasing respiration rate during oxidative assimilation of different substrates by resting Klebsiella aerogenes in a system open to oxygen. Biochemical Journal 136:1097–1104
     [Google Scholar]
  14. Downie J. A., Cox G. B. 1978; Sequence of bcytochromes relative to ubiquinone in the electron transport chain of Escherichia coli. Journal of Bacteriology 133:477–484
     [Google Scholar]
  15. Garland P. B., Downie J. A., Haddock B. A. 1975; Proton translocation and the respiratory nitrate reductase ofEscherichia coli. Biochemical Journal 152:547–559
     [Google Scholar]
  16. Gibson J. F., Hughes M. N., Poole R. K. 1979; Inhibition of growth and energy metabolism of Escherichia coli by triorganolead compounds. Society for General Microbiology Quarterly 6:74
     [Google Scholar]
  17. Gould J. M. 1976; Inhibition by triphenyltin chloride of a tightly-bound membrane component involved in phosphorylation. European Journal of Biochemistry 62:567–575
     [Google Scholar]
  18. Haddock B. A., Downie J. A., Garland P. B. 1976; Kinetic characterization of the membrane- bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual wavelength spectrophotometer. Biochemical Journal 154:285–294
     [Google Scholar]
  19. Kahn J. S. 1968; Chlorotri-n-butyltin. An inhibitor of phosphorylation in isolated chloroplasts. Biochimica et biophysica acta 153:203–210
     [Google Scholar]
  20. Leow A. C. T., Leaver D. D. 1977; Effect of triethyltin on Escherichia coli K12. Chemico Biological Interactions 19:339–351
     [Google Scholar]
  21. Mitchell P. 1963; Molecule, group and electron translocation through natural membranes. Biochemical Society Symposia 22:142–168
     [Google Scholar]
  22. Mitchell P., Moyle J. 1969; Translocation of some anions, cations and acids in rat liver mitochondria. European Journal of Biochemistry 9:149–155
     [Google Scholar]
  23. Pirt S. J. 1967; A kinetic study of the mode of growth of surface colonies of bacteria and fungi. Journal of General Microbiology 47:181–197
     [Google Scholar]
  24. Poole R. K. 1977; The influence of growth substrate and capacity for oxidative phosphorylation on respiratory oscillations in synchronous cultures of Escherichia coli K12. Journal of General Microbiology 99:369–377
     [Google Scholar]
  25. Poole R. K., Haddock B. A. 1974; Energylinked reduction of nicotinamide-adenine dinucleotide in membranes derived from normal and various respiratory-deficient strains ofEscherichia coli. Biochemical Journal 144:77–85
     [Google Scholar]
  26. Rice C. W., Hempfling W. P. 1978; Oxygen-limited continuous culture and respiratory energy conservation in Escherichia coli. Journal of Bacteriology 134:115–124
     [Google Scholar]
  27. Rose M. S., Aldridge W. N. 1972; Oxidative phosphorylation: the effect of anions on the inhibition by triethyltin of various mitochondrial functions, and the relationship between this inhibition and binding of triethyltin. Biochemical Journal 127:51–59
     [Google Scholar]
  28. Selwyn M. J. 1976; Triorganotin compounds as ionophores and inhibitors of ion translocating ATPases. In Organotin Compounds: New Chemistry and Applications Advances in Chemistry Series 157 pp. 204–226 Zuckerman J. J. Edited by Washington: American Chemical Society;
     [Google Scholar]
  29. Selwyn M. J., Dawson A. P., Stockdale M., Gaines N. 1970; Chloride-hydroxide exchange across mitochondrial, erythrocyte and artificial lipid membranes mediated by trialkyl- and triphenyltin compounds. European Journal of Biochemistry 14:120–126
     [Google Scholar]
  30. Shipp W. S. 1972; Cytochromes of Escherichia coli. Archives of Biochemistry and Biophysics 150:459–472
     [Google Scholar]
  31. Stevens C. D., Feldhake C. J., Kehoe R. A. 1960; Isolation of triethyllead ion from liver after inhalation of tetraethyllead. Journal of Pharmacology and Experimental Therapeutics 128:90–94
     [Google Scholar]
  32. Stockdale M., Dawson A. P., Selwyn M. J. 1970; Effects of trialkyltin and triphenyltin compounds on mitochondrial respiration. European Journal of Biochemistry 15:342–351
     [Google Scholar]
  33. Tsuchiya T., Rosen B. P. 1975; Energy transduction in Escherichia coli. The role of the Mg2+ ATPase. Journal of Biological Chemistry 250:8409–8415
     [Google Scholar]
  34. Watling-Payne A. S., Selwyn M. J. 1970; Effect of some organometallic compounds on the permeability of chloroplast membranes. FEBS Letters 10:139–142
     [Google Scholar]
  35. Watling-Payne A. S., Selwyn M. J. 1974; Inhibition and uncoupling of photophosphorylation in isolated chloroplasts by organotin, organomercury and diphenyleneiodonium compounds. Biochemical Journal 142:65–74
     [Google Scholar]
  36. West I. C., Mitchell P. 1974; Proton/sodium ion antiport in Escherichia coli. Biochemical Journal 144:87–90
     [Google Scholar]
  37. Yamada J., Tatsuguchi K., Watanabe T. 1978; Effects of trialkyltin chlorides on microbial growth. Agricultural and Biological Chemistry 42:1167–1172
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-116-1-99
Loading
Effects of Trialkyllead Compounds on Growth, Respiration and Ion Transport in Escherichia coli K12
Microbiology 116, 99 (1980); https://doi.org/10.1099/00221287-116-1-99
/content/journal/micro/10.1099/00221287-116-1-99
/content/journal/micro/10.1099/00221287-116-1-99
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error