Summary: Cell-free glucosyltransferase of strain B13 (serotype ) exclusively synthesized water-insoluble glucan from sucrose. The insoluble glucan possessed strong glucan-associated glucosyltransferase activity even after extensive washing and lyophilization. Furthermore, cell-free glucosyltransferase became bound to heat-treated water-insciable glucan or to heat-treated B13 cells grown in Todd Hewitt broth, and the resulting glucan and cells adhered to a glass surface in the presence of exogenous sucrose. No other water-insoluble glucans bound significant quantities of glucosyltransferase. Glucan synthesis by free or glucan-bound glucosyltransferase was stimulated by low concentrations (1 to 5 mg ml) of isomaltose or water-soluble dextrans of various molecular weights, but higher concentrations (10 mg ml) inhibited glucan synthesis. The glucan synthesized in the presence of primer dextrans exhibited a reduced ability to adhere to a glass surface. Certain sugars such as maltose and fructose significantly lowered the yield of insoluble glucans. Preincubation of glucosyltransferase with the low molecular wieght dextran T10 increased subsequent binding to B13 insoluble glucan. whereas preincubation with higher molecular weight dextrans significantly inhibited the glucosyltransferase binding.


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