1887

Abstract

A high-resolution screening procedure for the selection of mutants was developed based on the ability of some strains of to produce pigment in the light. Out of 23000 colonies investigated, 17 auxotrophic mutants were isolated. Specific growth factor requirements for the mutants and the proportion of revertants were determined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-116-1-253
1980-01-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/116/1/mic-116-1-253.html?itemId=/content/journal/micro/10.1099/00221287-116-1-253&mimeType=html&fmt=ahah

References

  1. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of the American Chemical Society 70:4267–4269
    [Google Scholar]
  2. Holland K. T., Ratledge C. 1971; A procedure for selecting and isolating specific auxotrophic mutants of Mycobacterium smegmatis. Journal of General Microbiology 66:115–118
    [Google Scholar]
  3. Koníčková-Radochová M., Koníček J., Málek I. 1970; The study of mutagenesis in Mycobacterium phlei. Folia microbiclogica 15:88–102
    [Google Scholar]
  4. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  5. Messer W., Vielmetter W. 1965; High resolution colony staining for the detection of bacterial growth requirement mutants using naphthol azo-dye techniques. Biochemical and Biophysical Research Communications 21:182–186
    [Google Scholar]
/content/journal/micro/10.1099/00221287-116-1-253
Loading
/content/journal/micro/10.1099/00221287-116-1-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error