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A new haemolysin from Staphylococcus aureus produced opaque zones of haemolysis on horse blood agar but did not lyse equine erythrocytes suspended in phosphate-buffered saline. The haemolysin was not neutralized by normal rabbit serum and was distinct from α, β- and δ-haemolysins as well as human leucocidin. Partially purified preparations produced erythema when injected intradermally into rabbit skin.
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