1887

Abstract

The bacterium C was grown in a chemostat on methanol as sole source of carbon and energy. At a dilution rate of 0·1 h, other methanol-utilizing bacteria ( 1 and 135), when added separately at a steady state, became dominant in the fermenter and C was excluded. At a dilution rate of 0·3 h, however, C dominated and the other bacteria were excluded. When various bacteria unable to utilize methanol were added to the chemostat during a steady state growth of C, they remained in the fermenter independent of the dilution rate, but as a very low percentage of the total population (about 1%). When pathogenic bacteria ( and ), which are unable to utilize methanol as a sole carbon source, were added separately to a pure culture of C in a chemostat, they too remained in the fermenter independent of the dilution rate. However, they constituted less than 1% of the population in the culture broth but a high percentage of the population on the fermenter wall. When added to a mixture of C and bacteria unable to utilize methanol, the pathogenic bacteria could not be found in the fermenter after a few medium changes.

The results suggest that operation of a continuous culture of C at high dilution rates serves to prevent contamination with other methylotrophs that may have lower yields. A mixture of C and heterotrophs from soil is relatively resistant to invasion by pathogens.

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1980-01-01
2021-05-12
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References

  1. Ballerini D., Parlovar D., Lapeyronnie M., Sri K. 1977; Mixed culture of bacteria utilizing methanol for growth. European Journal of Applied Microbiology 4:11–19
    [Google Scholar]
  2. Chalfan Y., Mateles R.I. 1972; New pseudomonad utilizing methanol for growth. Applied Microbiology 23:135–140
    [Google Scholar]
  3. Chen B.Y., Hirt W., Lim C.H., Tsao G.T. 1977; Growth characteristics of a new methy- lomonad. Applied and Environmental Microbiology 33:269–274
    [Google Scholar]
  4. Cowan S.T., Steel K.J. 1965 Manual for the Identification of Medical Bacteria pp. 55 and 76. Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Cremieux A., Chevalier J., Combet M., Bumenil G., Parlovar D., Ballerini D. 1977; Mixed culture of bacteria utilizing methanol for growth. European Journal of Applied Microbiology 4:1–9
    [Google Scholar]
  6. Goldberg I., Mateles R.I. 1975; Growth of Pseudomonas C on C1-compounds : a correction. Journal of Bacteriology 124:1028–1029
    [Google Scholar]
  7. Goldberg I., Rock J.S., Een-Bassat A., Mateles R.I. 1976; Bacterial yields on methanol, methylamine, formaldehyde and formate. Biotechriology and Bioengineering 18:16571668
    [Google Scholar]
  8. Häggström L. 1969; Studies on methanol oxidizing bacteria. Biotechnolugy and Bioengineering 11:1043–1054
    [Google Scholar]
  9. Häggström L. 1977; Mutant of Methylomonas methanolica and its characterization with respect to biomass production from methanol. Applied and Environmental Biology 33:567–576
    [Google Scholar]
  10. Harrison D.E.F. 1978; Mixed cultures in industrial fermentation processes. Advances in Applied Microbiology 24:129–164
    [Google Scholar]
  11. Harrision D.E.F., Wilkinson T.C., Wren S.J., Harwood J.H. 1976; Mixed bacterial cultures as a basis for continuous production of single cell protein from C1-compounds. In Continuous Culture 6. Applications and New Fields pp. 122–134 Dean A.C.R., Ellwood D.C., Evans C.G.T., Melling J. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  12. Linton J.D., Buckee J.C. 1977; Interactions in a methane-utilizing mixed bacterial culture in a chemostat. Journal of General Microbiology 101:219–225
    [Google Scholar]
  13. MacLennan D.G., Ousby J.C., Owen T.R., Steer D.C. 1974; Microbiological production of protein. British Patent No. 1370 892.
    [Google Scholar]
  14. Mateles R.I., Battat E. 1974; Continuous culture used for media optimization. Applied Microbiology 28:901–905
    [Google Scholar]
  15. Mateles R.I., Goldberg I., Battat E. 1976; Production of single cell protein from methanol. U.S. Patent No. 3, 989, 595
    [Google Scholar]
  16. Munson R.J., Bridges B.A. 1964; ‘Take-over’ -an unusual selection process in steady-state cultures of Escherichia coli.. Journal of General Microbiology 37:411–418
    [Google Scholar]
  17. Rock J.S., Goldberg I., Ben-Bassat A., Mateles R.I. 1976; Isolation and characterization of two methanol utilizing bacteria. Agricultural and Biological Chemistry 40:2129–2135
    [Google Scholar]
  18. Rokem J.S., Goldberg I., Mateles R.I. 1978; Maintenance requirement for bacteria growing on C1-compounds. Biotechnology and Bioengineering 20:1557–1564
    [Google Scholar]
  19. Snedecor B., Cooney C.L. 1974; Thermophilic mixed culture of bacteria utilizing methanol for growth. Applied Microbiology 27:1112–1117
    [Google Scholar]
  20. Stieglitz B., Mateles R.I. 1973; Methanol metabolism in Pseudomonas C. Journal of Bacteriology 114:390–398
    [Google Scholar]
  21. Terui G., Takada N., Sawada H. 1973; Methanol assimilating propagation of microbial cells. U.S. Patent No. 3, 755, 082
    [Google Scholar]
  22. Topiwala H.H., Hamer G. 1971; Effect of wall growth in steady-state continuous cultures. Biotechnology and Bioengineering 13:919–922
    [Google Scholar]
  23. Wilkinson T.G., Topiwala H.H., Hamer G. 1974; Interactions in a mixed bacterial population growing on methane in a continuous culture. Biotechnology and Bioengineering 16:41–59
    [Google Scholar]
  24. Wølfel R., Sontheimer H. 1974; Ein neues Verfahren zur Bestimmung von Organisch gebun- denen KohlenstofF in Wasser durch photochemi- sche Oxidation. Wasser 43:315–324
    [Google Scholar]
  25. Wren S.J. 1978 Ph.D. thesis; University of London:
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