Summary: In C, a facultative methylotrophic bacterium, methanol is assimilated via the 2-keto-3-deoxy-6-phosphogluconate (KDPG) variant of the ribulose monophosphate (RMP) pathway of formaldehyde fixation. The oxidation of methanol to CO is accomplished by the direct oxidation pathway (which involves formic acid as an oxidation intermediate), via a cyclic oxidation pathway (glucose monophosphate shunt) and by other decarboxylation reactions. The distribution pattern of methanol carbon among the assimilation and the different oxidation pathways was studied by measuring the distribution between CO and cell constituents of C-labelled compounds after their injection into a culture growing on methanol in a chemostat. From these measurements, it was calculated that 25% of the methanol consumed by the cells was oxidized through formate to CO, while the remainder was diverted into the hexulosephosphate synthase reaction from which 55% was assimilated through the KDPG reaction and 17% was oxidized to CO via a cyclic oxidation pathway and other decarboxylation reactions. The remaining 7% from the methanol carbon was re-incorporated as CO, into cell material through carboxylation reactions.


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