1887

Abstract

In C, a facultative methylotrophic bacterium, methanol is assimilated via the 2-keto-3-deoxy-6-phosphogluconate (KDPG) variant of the ribulose monophosphate (RMP) pathway of formaldehyde fixation. The oxidation of methanol to CO is accomplished by the direct oxidation pathway (which involves formic acid as an oxidation intermediate), via a cyclic oxidation pathway (glucose monophosphate shunt) and by other decarboxylation reactions. The distribution pattern of methanol carbon among the assimilation and the different oxidation pathways was studied by measuring the distribution between CO and cell constituents of C-labelled compounds after their injection into a culture growing on methanol in a chemostat. From these measurements, it was calculated that 25% of the methanol consumed by the cells was oxidized through formate to CO, while the remainder was diverted into the hexulosephosphate synthase reaction from which 55% was assimilated through the KDPG reaction and 17% was oxidized to CO via a cyclic oxidation pathway and other decarboxylation reactions. The remaining 7% from the methanol carbon was re-incorporated as CO, into cell material through carboxylation reactions.

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/content/journal/micro/10.1099/00221287-116-1-213
1980-01-01
2021-05-07
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References

  1. Anthony C. 1977; The prediction of growth yields in methylotrophs. Journal of General Microbiology 104:91–104
    [Google Scholar]
  2. Babel W., Loffhagen N. 1977; Carboxylierung von Phosphoenolpyruvate und Pyruvate durch das obligat methylotrophe Bakterium Pseudomonas W6. Zeitschrift für allgemeine Mikrobiologie 17:75–79
    [Google Scholar]
  3. Battat E., Goldberg I., Mateles R.I. 1974; Growth of Pseudomonas C on C1 compounds: continuous culture. Applied Microbiology 28:906–911
    [Google Scholar]
  4. Ben-Bassat A., Goldberg I. 1977; Oxidation of C1-compounds in Pseudomonas C. Biochimica et biophysica acta 497:586–597
    [Google Scholar]
  5. Bray G.A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  6. Chalfan Y., Mateles R.I. 1972; New pseudomonad utilizing methanol for growth. Applied Microbiology 23:135–140
    [Google Scholar]
  7. Colby J., Zatman L.J. 1975; Tricarboxylic acid-cycle and related enzymes in restricted facultative methylotrophs. Biochemical Journal 148:505–511
    [Google Scholar]
  8. van Dijken J.P., Harder W. 1975; Growth yields of microorganisms on methanol and methane. A theoretical study. Biotechnology and Bioengineering 17:15–30
    [Google Scholar]
  9. Goldberg I., Rock J.S., Ben-Bassat A., Mateles R.I. 1976; Bacterial yields on methanol, methylamine, formaldehyde and formate. Biotechnology and Bioengineering 18:1657–1668
    [Google Scholar]
  10. Ling K.H., Paetkau V., Markus F., Lardy H.A. 1966; Phosphofructokinase I. Skeletal muscle. Methods in Enzymology 9:425–429
    [Google Scholar]
  11. Mateles R.I., Battat E. 1974; Continuous culture used for media optimization. Applied Microbiology 28:901–905
    [Google Scholar]
  12. Miethe D., Babel N. 1976; Regulation der Glucose-6-phosphate Dehydrogenase aus dem obligat methylotrophen Bakterium Pseudomonas W6. Zeitschrift für allgemeine Mikrobiologie 16:289–299
    [Google Scholar]
  13. Quayle J.R. 1961; Metabolism of C1 compounds in autotrophic and heterotrophic microorganisms. Annual Review of Microbiology 15:119–152
    [Google Scholar]
  14. Quayle J.R. 1972; The metabolism of C1 compounds by microorganisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  15. Ribbons D.W., Harrison J.E., Wadzinsky A.M. 1970; Metabolism of single carbon compounds. Annual Review of Microbiology 24:135–158
    [Google Scholar]
  16. Stieglitz B., Mateles R.I. 1973; Methanol metabolism in Pseudomonas C. Journal of Bacteriology 114:390–398
    [Google Scholar]
  17. Strøm T., Ferenci T., Quayle J.R. 1974; The carbon assimilation pathways of Methylococcus capsulatus, Pseudomonas methanica and Methylosinus trichosporium (OB3B) during growth on methane. Biochemical Journal 144:465–476
    [Google Scholar]
  18. Taylor I.J. 1977; Carbon assimilation and oxidation by Methylophilus methylotrophus - the ICI SCP organism. In Microbial Growth on C-r Compounds: Abstracts of the International Symposium pp. 52–54 Pushchino: USSR Academy of Science;
    [Google Scholar]
  19. Taylor R.T., Weissbach H. 1965; Radioactive assay for serine transhydroxymethylase. Analytical Biochemistry 13:80–84
    [Google Scholar]
  20. Wiame J.M., Bourgeois S. 1955; Le role de l’anhydride carbonique dans les croissances bacteriennes. Biochimica et biophysica acta 18:269–278
    [Google Scholar]
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