1887

Abstract

[C]Methylamine was transported into mycelial cells of by a specific and energy-dependent transport system having an optimum at pH 6·0, a K of 12·5 and a of 4·1 mol (g dry wt) min; uptake occurred against a concentration gradient. NH competitively inhibited methylamine transport with higher affinity towards the latter system. Sucrose and nitrate were required during transport for maximal activity. Highest transport activity developed in nitrate-grown mycelium. Nitrogen starvation decreased the activity by approximately 60%. Preloading of mycelium with glutamine, asparagine or ammonia almost completely prevented methylamine uptake. Transport activity was inversely proportional to the intracellular concentration of the -amides. It is postulated that ammonia uptake might be regulated by -amides rather than by ammonia.

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/content/journal/micro/10.1099/00221287-116-1-201
1980-01-01
2021-10-26
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