Isolation of a Methanol Dehydrogenase with a Functional Coupling to Cytochrome c

Summary: A methanol dehydrogenase containing cytochrome c which was reduced on addition of methanol was isolated from Hyphomicrobium X. Metal-chelators or oxygen blocked this electron transport. Oxygen changed the coupling irreversibly, transforming the enzyme in the complex into the already known, NH4 +-requiring enzyme form. This ‘classical’ methanol dehydrogenase differs in several respects from the more natural enzyme reported here.