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A methanol dehydrogenase containing cytochrome c which was reduced on addition of methanol was isolated from Hyphomicrobium X. Metal-chelators or oxygen blocked this electron transport. Oxygen changed the coupling irreversibly, transforming the enzyme in the complex into the already known, NH4 +-requiring enzyme form. This ‘classical’ methanol dehydrogenase differs in several respects from the more natural enzyme reported here.
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