Preparation of Protoplasts and Whole Cell Ghosts from Free

Abstract

Cell wall-deficient forms of were produced in growth medium containing -cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).

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/content/journal/micro/10.1099/00221287-115-2-517
1979-12-01
2024-03-29
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