RT Journal Article SR Electronic(1) A1 Rapp, Peter A1 Bock, Hans A1 Wray, Victor A1 Wagner, FritzYR 1979 T1 Formation, Isolation and Characterization of Trehalose Dimycolates from Rhodococcus erythropolis Grown on n-Alkanes JF Microbiology, VO 115 IS 2 SP 491 OP 503 DO https://doi.org/10.1099/00221287-115-2-491 PB Microbiology Society, SN 1465-2080, AB Rhodococcus erythropolis DSM 43215 produced a surface-active trehalose lipid whose formation was induced by n-alkanes to a maximum of 2*1 g l−1 in a 501 batch culture on 2% (w/v) «-alkanes of chain length C12 to C18. The glycolipid was extracted from the biomass with n-hexane and was purified by repeated chromatography on silica gel. It contained a,a-trehalose as the sole non-reducing sugar. The lipid moiety was characterized by 13C nuclear magnetic resonance spectroscopy and mass spectrometry and consisted predominantly of saturated long-chain a-branched ^-hydroxy fatty acids (mycolic acids) ranging from C32H6403 to C38H7603, of which C34H6803 and C35H70O3 predominated. The molar ratio of trehalose to mycolic acids was 1:2. 13C nuclear magnetic resonance analysis of the O- hexamethyltrehalose obtained by saponification of the permethylated trehalose dimycolates revealed, with the aid of deuterium exchange, that the ester linkages of mycolic acids are to both primary alcohol groups at the C-6 and C-6' positions of the trehalose., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-115-2-491