1887

Abstract

Several strains of agar-degrading bacteria were isolated from the River Wey, Guildford, during the summer of 1976. All were Gram-negative rods and could be divided into two groups. Those which only softened the agar belonged to the genus and those which caused extensive liquefaction of the agar have been referred to the genus . Attempts to isolate, purify and characterize the enzymes showed some differences between the two taxa. The strains of produced at least two enzyme complexes, one cell-free and the other cell-bound, and hydrolysed agar with the formation of oligosaccharides. The strains of , on the other hand, readily released ‘agarase’ into the medium yielding monosaccharides as major end-products. The agar-degrading enzymes of both groups were inducible, not only by agar, but also by other galactans and polysaccharides associated with plants. The enzyme preparations also hydrolysed a wide range of plant-derived polysaccharides, including some associated with terrestrial and freshwater plants rather than with marine algae. These results suggest that there is no special ecological reason for the presence of agarolytic bacteria in fresh water but that their activity reflects the wide substrate spectrum of the polysaccharides of such organisms. The agar-softening strains are considered to belong to a new species for which the name is proposed.

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1979-12-01
2021-10-17
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References

  1. Araki C. 1937; Chemical studies of agar-agar. III. Acetylation of agar-like substances from Gelidium amansii . Journal of the Chemical Society of Japan 58:1338–1350
    [Google Scholar]
  2. Araki C. 1956; Structures of agarose constituents of agar-agar. Bulletin of the Chemical Society of Japan 29:543–544
    [Google Scholar]
  3. Bergey D. H., Breed R. S., Hammer B. W., Huntoon F. M., Murray E. G. D., Harrison F. C. 1934 Bergey’s Manual of Determinative Bacteriology, 4th edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Buchanan R. E., Gibbons N. E. 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Christensen P. J. 1977a; Synonymy of Flavobacterium pectinovorum Dorey with Cytophaga johnsonae Stanier. International Journal of Systematic Bacteriology 27:122–132
    [Google Scholar]
  6. Christensen P. J. 1977b; The history, biology and taxonomy of the Cytophaga group. Canadian Journal of Microbiology 23:1599–1653
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria, 2nd edn.. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. De Ley J. 1970; Re-examination of the association between melting points, buoyant density and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738–754
    [Google Scholar]
  9. Dorey M. J. 1959; Some properties of a pectolytic soil flavobacterium. Journal of General Microbiology 20:91–104
    [Google Scholar]
  10. Duckworth M., Yaphe W. 1970; Thin-layer chromatographic analysis of enzymic hydrolysates of agar. Journal of Chromatography 49:482–487
    [Google Scholar]
  11. Duckworth M., Yaphe W. 1971a; The structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohydrate Research 16:189–197
    [Google Scholar]
  12. Duckworth M., Yaphe W. 1971b; The structure of agar. II. The use of bacterial agarase to elucidate structural features of the charged polysaccharide in agar. Carbohydrate Research 16:435–445
    [Google Scholar]
  13. Dygert S., Li L., Florida D., Thoma J. A. 1965; Determination of reducing sugar with improved precision. Analytical Biochemistry 13:367–374
    [Google Scholar]
  14. Follett E. A. C., Webley D. M. 1965; An electron microscope study of the cell surface of Cytophaga johnsonae and some observations on related organisms. Antonie van Leeuwenhoek 31:361–382
    [Google Scholar]
  15. Gran H. H. 1902; Studien über Meeresbakterien. II. Ueber die Hydrolysis des Agar durch ein neues Enzyme die Gelase. Bergen Museums Aarbog, No. 2. [Cited in STANIER, R. Y. (1941). Studies on marine agar-digesting bacteria. Journal of Bacteriology 42:527–559
    [Google Scholar]
  16. Gray P. H. H., Chalmers C. H. 1924; On the stimulating action of certain organic compounds on cellulose decomposition by means of a new aerobic micro-organism that attacks both cellulose and agar. Annals of Applied Biology 11:324–338
    [Google Scholar]
  17. v. Hofsten B., Malmqvist M. 1975; Degradation of agar by a Gram-negative bacterium. Journal of General Microbiology 87:150–158
    [Google Scholar]
  18. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative meta-bolism of carbohydrates by various Gram-negative bacteria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  19. Hunger W., Claus D. 1978; Reisolation and growth of Bacillus agar-exedens . Antonie van Leeuwenhoek 44:105–113
    [Google Scholar]
  20. Izumi K. 1972; Chemical heterogeneity of the agar from Gracilaria verrucosa . Journal of Biochemistry 72:135–140
    [Google Scholar]
  21. Jeffries C. D., Holtman D. F., Guse D. G. 1957; Rapid method for determining the activity of micro-organisms on nucleic acids. Journal of Bacteriology 73:590–591
    [Google Scholar]
  22. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44:301
    [Google Scholar]
  23. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature; London: 178703
    [Google Scholar]
  24. Laycock R. H., Regier L. W. 1971; Trimethyl-amine-producing bacteria on haddock (Melanor-gramas aeglifinus) fillets during refrigerated storage. Journal of the Fisheries Research Board of Canada 28:305–307
    [Google Scholar]
  25. Lee J. V., Gibson D. M., Shewan J. M. 1977; A numerical taxonomic study of some pseudomonas-like marine bacteria. Journal of General Microbiology 98:439–451
    [Google Scholar]
  26. Lewin R. A. 1969; A classification of flexibacteria. Journal of General Microbiology 58:189–206
    [Google Scholar]
  27. Mandel M., Leadbetter E. R., Pfennig N., Truper H. G. 1971; Deoxyribonucleic acid base composition of phototrophic bacteria. International Journal of Systematic Bacteriology 21:222–230
    [Google Scholar]
  28. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  29. Mcquitty L. L. 1967; A mutual development of some typological theories and pattern-analytic methods. Educational and Psychological Measurement 27:21–46
    [Google Scholar]
  30. van der Meulen H. J. 1975 The enzymic hydrolysis of agar by Cytophaga fievensis sp.nov Ph.D thesis University of Groningen, The Netherlands:
    [Google Scholar]
  31. van der Meulen H. J., Harder W. 1975; Production and characterization of the agarase of Cytophaga fievensis . Antonie van Leeuwenhoek 41:431–447
    [Google Scholar]
  32. van der Meulen H. J., Harder W. 1976a; The regulation of agarase production by resting cells of Cytophaga fievensis . Antonie van Leeuwenhoek 42:277–286
    [Google Scholar]
  33. van der Meulen H. J., Harder W. 1976b; Characterization of the neoagarotetraase and neoagarobiase of Cytophaga fievensis . Antonie van Leeuwenhoek 42:81–94
    [Google Scholar]
  34. van der Meulen H. J., Harder W., Veldkamp H. 1974; Isolation and characterization of Cytophaga fievensis sp.n., a new agarolytic flexibacterium. Antonie van Leeuwenhoek 40:329–346
    [Google Scholar]
  35. Mitchell T. G., Hendrie M. S., Shewan J. M. 1969; The taxonomy, differentiation and identi-fication of Cytophaga species. Journal of Appliea Bacteriology 32:40–50
    [Google Scholar]
  36. Møller V. 1955; Simplified tests for amino acid decarboxylases and for the arginine hydrolase system. Acta pathologica et microbiologica scandinavica 36:158–172
    [Google Scholar]
  37. Ng Ying Kin N. M. K., Yaphe W. 1972; Properties of agar: parameters affecting gel formation and the agarase-iodine reaction. Carbohydrate Research 25:379–385
    [Google Scholar]
  38. Nicol H. 1931; Distribution of agar-liquefying bacteria. Nature; London: 1281041–1042
    [Google Scholar]
  39. Owen R. J., Lapage S. P. 1974; A comparison of strains of King’s Group IIb of Flavobacterium with Flavobacterium meningosepticum . Antonie van Leeuwenhoek 40:255–264
    [Google Scholar]
  40. Shewan J. M., Hobbs G., Hodgkiss W. 1960; A determinative scheme for the identification of certain genera of Gram-negative bacteria with special reference to the Pseudomonadaceae. Journal of Applied Bacteriology 23:379–390
    [Google Scholar]
  41. Sneath P. H. A. 1957; The application of computers to taxonomy. Journal of General Microbiology 17:201–226
    [Google Scholar]
  42. Stanier R. Y. 1942; The Cytophaga group: a contribution to the biology of myxobacteria. Bacteriological Reviews 6:143–196
    [Google Scholar]
  43. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. Journal of Applied Bacteriology 23:37–52
    [Google Scholar]
  44. Turvey J. R., Christison J. 1967; The hydrolysis of algal galactans by enzymes from a Cytophaga species. Biochemical Journal 105:311–316
    [Google Scholar]
  45. Vattuone M. A., de Flores E. A., Sampietro A. R. 1975; Isolation of neoagarobiose and neoagarotetraose from agarose digested by Pseudomonas elongata . Carbohydrate Research 39:164–167
    [Google Scholar]
  46. Wishart D. 1969; Fortran II programs for 8 methods of cluster analysis. Computer Contribution 38 State Geological Survey, University of Kansas, Lawrence, U.S.A:
    [Google Scholar]
  47. Yaphe W. 1966; The purification and properties of an agarase from a marine bacterium, Pseudomonas atlantica . Proceedings of the 5th International Seaweed Symposium pp. 333–335 Oxford: Pergamon Press;
    [Google Scholar]
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