1887

Abstract

Homogenates of exhibited a Mg-dependent adenosine triphosphatase (ATPase) activity, with a pH optimum in Tris buffers of 8·2 to 8·3. The activity was not sensitive to oxygen. At high concentrations, quercetin and 4-chloro-7-nitrobenzofurazan inhibited ATPase activity in the cytoplasmic extract by 20 and 70%, respectively, whereas oligomycin, venturicidin, triethyltin, leucinostatin, dibutylchloromethyltin chloride, spegazzinine, efrapeptin, citreoviridin and sodium azide had no effect and -dicyclohexylcarbodiimide stimulated the activity somewhat. The activity was localized in a population of small cytoplasmic particles which also contained an acid phosphatase. There was no indication of an association of ATPase with hydrogenosomes. The ATPase activity (or activities) in this aerotolerant anaerobe is different from the ATPases characteristic of mitochondria or of anaerobic bacteria.

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1979-12-01
2024-04-19
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References

  1. Brugerolle G. 1972; Characterisation ultra-structurale et cytochimique de 2 types de granules cytoplasmiques chez les Trichomonas . Protistologica 8:352–363
    [Google Scholar]
  2. Cartledge T. G., Lloyd D. 1973; Changes in enzyme activities and distributions during glucose derepression and respiratory adaptation of anaerobically grown Saccharomyces carlsbergensis . Biochemical Journal 132:609–621
    [Google Scholar]
  3. Čerkasov J., Čerkasovová A., Kulda J., Vilhelmová D. 1978; Respiration of hydrogenosomes of Tritrichomonas foetus. I. ADP-dependent oxidation of malate and pyruvate. Journal of Biological Chemistry 253:1207–1214
    [Google Scholar]
  4. Čerkasovová A. 1970; Energy-producing metabolism of Tritrichomonas foetus. I. Evidence for control of intensity and the contribution of aerobiosis to total energy production. Experimental Parasitology 27:165–178
    [Google Scholar]
  5. Čerkasovová A., Čerkasov J. 1976; Some properties of the membrane of hydrogenosomes of Tritrichomonas foetus . In Biochemistry of Parasites and Host-Parasite Relationships pp. 23–30 Van den Bossche H. Edited by Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  6. Clarke D., Morris J. G. 1976; Partial purification of DCCD-sensitive membrane ATPase complex from the obligately-anaerobic bacterium Clostridium pasteurianum . Biochemical Journal 154:725–729
    [Google Scholar]
  7. Clarke D., Morris J. G. 1977; Reconstitution of a functional proton-translocating adenosine triphosphatase from the obligately-anaerobic bacterium Clostridium pasteurianum . Biochemical Society Transactions 5:140–143
    [Google Scholar]
  8. Daniel W. A., Mattern C. F. T., Honigberg B. M. 1971; Fine structure of the mastigont system in Tritrichomonas muris (Grassi). Journal of Protozoology 18:575–586
    [Google Scholar]
  9. Diamond L. S. 1957; The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43:488–490
    [Google Scholar]
  10. De Duve C. 1967; General principles. In Enzyme Cytology pp. 1–26 Roodyn D. B. Edited by New York: Academic Press;
    [Google Scholar]
  11. Honigberg B. M. 1978; Trichomonads of veterinary importance. In Parasitic Protozoa 2 pp. 163–273 Kreier J. P. Edited by New York: Academic Press;
    [Google Scholar]
  12. Honigberg B. M., Mattern C. F. T., Daniel W. A. 1971; Fine structure of the mastigont system in Tritrichomonas foetus (Riedmuller). Journal of Protozoology 18:183–198
    [Google Scholar]
  13. Leighton F., Poole B., Beaufay H., Baudhuin P., Coffey J. W., Fowler S., De Duve C. 1968; The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. Journal of Cell Biology 37:482–513
    [Google Scholar]
  14. Leloir L. F., Cardini C. E. 1957; Characterization of phosphorus compounds by acid lability. Methods in Enzymology 3:841–850
    [Google Scholar]
  15. Lindmark D. G., Müller M. 1973; Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate, Tritrichomonas foetus, and its role in pyruvate metabolism. Journal of Biological Chemistry 248:7724–7728
    [Google Scholar]
  16. Lindmark D. G., Müller M., Shio H. 1975; Hydrogenosomes in Trichomonas vaginalis . Journal of Parasitology 61:552–554
    [Google Scholar]
  17. Lloyd D., Edwards S. W. 1976; Mitochondrial adenosine triphosphatase of the fission yeast Schizosaccharomyces pombs 972h. Changes in activity and inhibitor sensitivity in response to catabolite repression. Biochemical Journal 160:335–342
    [Google Scholar]
  18. Lloyd D., Edwards S. W. 1977; Mitochondrial adenosine triphosphatase of the fission yeast Schizosaccharomyces pombe 972h. Change in activity and inhibitor sensitivities during the cell cycle indicate similarities and differences in binding sites. Biochemical Journal 162:581–590
    [Google Scholar]
  19. Lloyd D., Lindmark D. G., Müller M. 1979; Respiration of Tritrichomonas foetus: absence of detectable cytochromes. Journal of Parasitology 65:466–469
    [Google Scholar]
  20. Müller M. 1973; Biochemical cytology of trichomonad flagellates. I. Subcellular localization of hydrolases, dehydrogenases, and catalase in Tritrichomonas foetus . Journal of Cell Biology 57:453–474
    [Google Scholar]
  21. Müller M., Lindmark D. G. 1978; Respiration of hydrogenosomes in Tritrichomonas foetus. II. Effect of CoA on pyruvate oxidation. Journal of Biological Chemistry 253:1215–1218
    [Google Scholar]
  22. Opperdoes F. R., Borst P., Spits H. 1977; Particle-bound enzymes in the bloodstream form of Trypanosoma brucei . European Journal of Biochemistry 76:21–28
    [Google Scholar]
  23. Racker E. 1976 In A New Look at Mechanisms in Bioenergetics p. 168 New York: Academic Press;
    [Google Scholar]
  24. Riebeling V., Thauer R. K., Jungermann K. 1975; The internal-alkaline pH gradient sensitive to uncoupler and ATPase inhibitor, in growing Clostridium pasteurianum . European Journal of Biochemistry 55:445–453
    [Google Scholar]
  25. Ryley J. F. 1955; Studies on the metabolism of the protozoa. 5. Metabolism of the parasitic flagellate Trichomonas foetus . Biochemical Journal 59:361–363
    [Google Scholar]
  26. Uyeda K., Rabinowitz J. G. 1971; Pyruvate-ferredoxin oxidoreductase of Clostridium acidiurici . Journal of Biological Chemistry 246:3111–3119
    [Google Scholar]
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