1887

Abstract

Fifteen independent menaquinone biosynthesis mutants () of K12, selected for their inability to use fumarate as terminal electron acceptor, were investigated. Two nutritionally distinct groups were detected. The major group (13 mutants) responded to 1,4-dihydroxy-2-naphthoate (DHN), 2-succinylbenzoate (SB) and its dilactone, whereas the minor group (2 mutants) only responded to DHN. DHN was at least five times more effective than SB but it inhibited growth at concentrations greater than 10 . For anaerobic growth on glucose minimal medium the auxotrophs responded to much lower concentrations of DHN and SB and these intermediates could be replaced by uracil. Anaerobic growth tests showed that glycerol, formate and H are good substrates for when fumarate is the ultimate electron acceptor but growth with lactate or with fumarate alone is poor.

All 15 mutations were located between and at approximately 49 min in the linkage map. Cotransduction frequencies with relevant markers were: (21%), (35%) and (15%). The presence of at least three genetically distinct classes ( and , SB-requirers; , DHN-requirers) was indicated using abortive transduction as a complementation test and three-factor genetic analysis. The relative orientation was indicated.

Fluoroacetate-resistant mutants were isolated and four different classes were identified: , lacking acetate kinase; , lacking phosphotransacetylase; , lacking both of these activities; and , which retained both of these enzyme activities. Some of the mutants and all of the mutants failed to grow on media containing fumarate as terminal electron acceptor or anaerobically on glucose minimal medium. All four types had genetic lesions clustered between the and sites. Average cotransduction frequencies with relevant markers were: (4%), (27 to 35%) and (71 to 80%).

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1979-12-01
2024-04-18
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References

  1. Bachmann B. J., Low K. B., Taylor A. L. 1976; Recalibrated linkage map of Escherichia coli K-12. Bacteriological Reviews 40:116–167
    [Google Scholar]
  2. Brown T. D. K., Jones-Mortimer M. C., Kornberg H. L. 1977; The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli . Journal of General Microbiology 102:327–336
    [Google Scholar]
  3. Bryant R. W., Bentley R. 1976; Menaquinone biosynthesis: conversion of o-succinylbenzoic acid to 1,4-dihydroxy-2-naphthoic acid and menaquinones by Escherichia coli extracts. Biochemistry 15:4792–4796
    [Google Scholar]
  4. Creaghan I. T., Guest J. R. 1978; Succinate dehydrogenase-dependent nutritional requirement for succinate in mutants of Escherichia coli K12. Journal of General Microbiology 107:1–13
    [Google Scholar]
  5. Gottschalk G., Andreesen J. R. 1979; Energy metabolism in anaerobes. In International Review of Biochemistry 21 Microbial Biochemistry pp. 85–115 Quayle J. R. Edited by Baltimore: University Park Press;
    [Google Scholar]
  6. Guest J. R. 1977; Menaquinone biosynthesis: mutants of Escherichia coli K-12 requiring 2-succinylbenzoate. Journal of Bacteriology 130:1038–1046
    [Google Scholar]
  7. Haddock B. A., Jones C. W. 1977; Bacterial respiration. Bacteriological Reviews 41:47–99
    [Google Scholar]
  8. Hayashi S., Koch J. P., Lin E. C. C. 1964; Active transport of l-α-glycerophosphate in Escherichia coli . Journal of Biological Chemistry 239:3098–3105
    [Google Scholar]
  9. Jacobs N. J., Jacobs J. M. 1978; Quinones as hydrogen carriers for a late step in anaerobic heme biosynthesis in Escherichia coli . Biochimica et biophysica acta 544:540–546
    [Google Scholar]
  10. Kröger A. 1977; Phosphorylative electron transport with fumarate and nitrate as terminal hydrogen acceptors. Symposia of the Society for General Microbiology 27:61–93
    [Google Scholar]
  11. Kröger A. 1978; Fumarate as terminal acceptor of phosphorylative electron transport. Biochimica et biophysica acta 505:129–145
    [Google Scholar]
  12. Lambden P. R., Guest J. R. 1976; Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor. Journal of General Microbiology 97:145–160
    [Google Scholar]
  13. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
    [Google Scholar]
  14. Low K. B. 1972; Escherichia coli F-prime factors, old and new. Bacteriological Reviews 36:587–607
    [Google Scholar]
  15. Newton N. A., Cox G. B., Gibson F. 1971; The function of menaquinones (vitamin K2) in Escherichia coli K-12. Biochimica et biophysica acta 244:155–166
    [Google Scholar]
  16. Shineberg B., Young I. G. 1976; Biosynthesis of bacterial menaquinone: the membrane-associated 1,4-dihydroxy-2-naphthoate octa-prenyltransferase of Escherichia coli . Biochemistry 15:2754–2758
    [Google Scholar]
  17. Spencer M. E., Guest J. R. 1973; Isolation and properties of fumarate reductase mutants of Escherichia coli . Journal of Bacteriology 114:563–570
    [Google Scholar]
  18. Spencer M. E., Lebeter V. M., Guest J. R. 1976; Location of the aspartase gene (aspA) on the linkage map of Escherichia coli K12. Journal of General Microbiology 97:73–82
    [Google Scholar]
  19. Young I. G. 1975; Biosynthesis of bacterial menaquinones. Menaquinone mutants of Escherichia coli . Biochemistry 14:399–406
    [Google Scholar]
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