1887

Abstract

A diaminopimelate-requiring mutant of was shown to lack the enzyme -acetyl--diaminopimelate deacylase. By repeated subculture of this mutant, a second-stage mutant was derived that could grow slowly in minimal defined medium supplemented with -diaminopimelate alone. After growth in such medium, the peptidoglycan of the wall still contained mainly and -diaminopimelate; radioactivity from -diamino-[C]pimelate entered peptidoglycan (as diamino[C]pimelate) and protein (as [C]lysine) to similar extents.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-115-1-95
1979-11-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/115/1/mic-115-1-95.html?itemId=/content/journal/micro/10.1099/00221287-115-1-95&mimeType=html&fmt=ahah

References

  1. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  2. Day A., White P. J. 1977; Enzymic assays for isomers of 2,6-diaminopimelic acid in walls of Bacillus cereus and Bacillus megaterium . Biochemical Journal 161:677–685
    [Google Scholar]
  3. Day A., White P. J. 1979; Uptake of individual isomers of 2,6-diaminopimelate and their incorporation into walls during growth of Bacillus megaterium . Journal of General Microbiology 110:401–407
    [Google Scholar]
  4. Park J. T., Hancock R. 1960; A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus . Journal of General Microbiology 22:249–258
    [Google Scholar]
  5. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviour of isomers of α,ε-diaminopimelic acid on paper chromatograms. Journal of the American Chemical Society 77:4844–4846
    [Google Scholar]
  6. Saleh F., White P. J. 1976; Use of auxotrophic mutants to isolate ll- or dd-isomers of 2,6-diaminopimelic acid. Journal of General Microbiology 96:253–261
    [Google Scholar]
  7. Schleifer K. H., Hammes W. P., Kandler O. 1976; Effect of endogenous and exogenous factors on the primary structures of bacterial peptidoglycan. Advances in Microbial Physiology 13:245–292
    [Google Scholar]
  8. Sundharadas G., Gilvarg C. 1967; Biosynthesis of α,ε-diaminopimelic acid in Bacillus megaterium . Journal of Biological Chemistry 242:3983–3984
    [Google Scholar]
  9. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools . Journal of General Microbiology 64:171–185
    [Google Scholar]
  10. Ward J. B. 1975; Peptidoglycan synthesis in L-phase variants of Bacillus licheniformis and Bacillus subtilis . Journal of Bacteriology 124:668–678
    [Google Scholar]
  11. White P. J. 1971; Diaminopimelate decarboxylase (Escherichia coli). Methods in Enzymology 17B:140–145
    [Google Scholar]
  12. White P. J. 1972; The nutrition of Bacillus megaterium and Bacillus cereus . Journal of General Microbiology 71:505–514
    [Google Scholar]
  13. White P. J., Lejeune B., Work E. 1969; Assay and properties of diaminopimelate epimerase from Bacillus megaterium . Biochemical Journal 113:589–601
    [Google Scholar]
  14. Wilkinson B. J., White P. J. 1973; The effect of antibiotics on synthesis of mucopeptide and teichoic acid by Pediococcus cerevisiae and by a substrain that requires methicillin for growth. Journal of General Microbiology 79:195–204
    [Google Scholar]
  15. Work E. 1957; Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of α,ε-diaminopimelic acid. Biochemical Journal 67:416–423
    [Google Scholar]
  16. Work E. 1962; Diaminopimelate decarboxylase. Methods in Enzymology 5:864–870
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-115-1-95
Loading
/content/journal/micro/10.1099/00221287-115-1-95
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error