1887

Abstract

During nitrogen deprivation, synthesis of glutamine synthetase was induced in non-growing conidia of When ammonia or glutamine was added to conidia which had been deprived of nitrogen, glutamine and arginine accumulated at a higher rate than in condia not deprived of nitrogen. The degradation of exogenous glutamine to glutamate is apparently a necessary step in the accumulation of glutamine and arginine within the conidia. In non-growing conidia, a cycle probably operates in which glutamine is degraded and resynthesized. The advantages of such a cycle would be that the carbon and nitrogen could be used to synthesize amino acids in general, as well as for the synthesis and accumulation of arginine and/or glutamine in particular.

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1979-11-01
2024-04-16
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References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Cooper A. J., Meister A. 1977; The glutamine transaminase-w-amidase pathway. CRC Critical Reviews in Biochemistry 4:281–303
    [Google Scholar]
  3. Cooper A. J., Stephani R. A., Meister A. 1976; Enzymatic reactions with methionine sulfoximine. Conversion to the corresponding α-imino and α-keto acids and to α-ketobutyrate and methane sulfinimide. Journal of Biological Chemistry 251:6674–6682
    [Google Scholar]
  4. Dávila G., Sánchez F., Palacios R., Mora J. 1978; Genetics and physiology of Neurospora crassa glutamine auxotrophs. Journal of Bacteriology 134:693–698
    [Google Scholar]
  5. Espín G., Mora J. 1978; Effect of the deprivation of amino acids on conidia of Neurospora crassa . Journal of General Microbiology 104:233–240
    [Google Scholar]
  6. Ferguson A. R., Sims A. P. 1974; The regulation of glutamine metabolism in Candida utilis: the inactivation of glutamine synthetase. Journal of General Microbiology 80:173–185
    [Google Scholar]
  7. Fincham J. R. S. 1950; Mutant strains of Neurospora deficient in animating ability. Journal of Biological Chemistry 182:61–73
    [Google Scholar]
  8. Monder C., Meister A. 1958; α-Ketoglutaramic acid as a product of enzymic trans-amination of glutamine in Neurospora . Biochimica et biophysica acta 28:202–203
    [Google Scholar]
  9. Mora Y., Espín G., Willms K., Mora J. 1978; Nitrogen accumulation in mycelium of Neurospora crassa . Journal of General Microbiology 104:241–250
    [Google Scholar]
  10. Palacios R. 1976; Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure. Journal of Biological Chemistry 251:4787–4791
    [Google Scholar]
  11. Palacios R., Campomanes M., Quinto C. 1977; Neurospora crassa glutamine synthetase. Translation of specific messenger ribonucleic acid in a cell-free system derived from rabbit reticulocytes. Journal of Biological Chemistry 252:3028–3034
    [Google Scholar]
  12. Quinto C., Mora J., Palacios R. 1977; Neurospora crassa glutamine synthetase. Role of enzyme synthesis and degradation during exponential growth. Journal of Biological Chemistry 252:8724–8727
    [Google Scholar]
  13. Ronzio R. A., Meister A. 1968; Phosphorylation of methionine sulfoximine by glutamine synthetase. Proceedings of the National Academy of Sciences of the United States of America 59:164–170
    [Google Scholar]
  14. Sánchez S., Martínez L., Mora J. 1972; Interactions between amino acid transport systems in Neurospora crassa . Journal of Bacteriology 112:276–284
    [Google Scholar]
  15. Schindler J., Sussman M. 1977; Ammonia determines the choice of morphogenic pathways in Dictyostelium discoideum . Journal of Molecular Biology 116:161–169
    [Google Scholar]
  16. Thayer S. P., Horowitz N. H. 1951; The l-amino acid oxidase of Neurospora . Journal of Biological Chemistry 192:755–767
    [Google Scholar]
  17. Vaca G., Mora J. 1977; Nitrogen regulation of arginase in Neurospora crassa . Journal of Bacteriology 131:719–725
    [Google Scholar]
  18. Van Pilsum J. F., Martin R. P., Kito E., Hess J. 1956; Determination of creatine, creatinine, arginine, guanido-acetic acid, guanidine and methyl-guanidine in biological fluids. Journal of Biological Chemistry 222:225–236
    [Google Scholar]
  19. Vichido I., Mora Y., Quinto C., Palacios R., Mora J. 1978; Nitrogen regulation of glutamine synthetase in Neurospora crassa . Journal of General Microbiology 106:251–259
    [Google Scholar]
  20. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
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