1887

Abstract

Spores of the fission yeast have been separated from vegetative cells by a simple and rapid centrifugation (800 for 20 min) through a 35% Hypaque solution to a purity >95%. Approximately 35% of the spores were recovered. Regrowth in EMM2 plus glucose showed that over 97% of the spores germinated within the first 2 h and outgrowth continued between 5 and 10 h. Sucrose induced germination in > 95% of the spores with a 1 h delay and outgrowth in 50 % of the spores with a 3 h delay. There was little protein synthesis during germination but the protein content increased linearly coincident with outgrowth. The RNA content increased slightly during germination, but increased linearly 1 h before the onset of outgrowth and protein synthesis. After 8 h of regrowth, coincident with the onset of DNA synthesis, the rate of RNA synthesis was accelerated. The DNA content had increased 1·7-fold after 10 h of regrowth from a haploid level of 1·36 10 g spore.

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/content/journal/micro/10.1099/00221287-115-1-255
1979-11-01
2021-08-05
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References

  1. Adelman M.R., Blobel G., Sabatini D.D. 1973; An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.. Journal of Cell Biology 56:191–205
    [Google Scholar]
  2. Allen P.J. 1965; Metabolic aspects of spore germination in fungi.. Annual Review of Phytopathology 13:313–342
    [Google Scholar]
  3. Bainbridge B.W. 1971; Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. . Journal of General Microbiology 66:319–325
    [Google Scholar]
  4. Blum J.J., Padilla G.M. 1962; Studies on synchronized cells: the time course of DNA, RNA, and protein synthesis in Astasia longa. . Experimental Cell Research 28:512–525
    [Google Scholar]
  5. Bostock C.J. 1970; DNA synthesis in the fission yeast Schizosaccharomyces pombe. . Experimental Cell Research 60:16–26
    [Google Scholar]
  6. Egel R. 1971; Physiological aspects of conjugation in fission yeast.. Planta 98:89–96
    [Google Scholar]
  7. Mitchison J.M. 1970; Physiological and cytological methods for Schizosaccharomyces pombe. . Methods in Cell Physiology 4:131–177
    [Google Scholar]
  8. Mitchison J.M., Creanor J. 1969; Linear synthesis of sucrase and phosphatases during the cell cycle of Schizosaccharomyces pombe. . Journal of Cell Science 5:373–391
    [Google Scholar]
  9. Nishi K., Shimoda C., Hayashibe M. 1978; Germination and outgrowth of Schizosaccharomyces pombe ascospores isolated by Urografin density gradient centrifugation.. Canadian Journal of Microbiology 24:893–897
    [Google Scholar]
  10. Padilla G.M., Creanor J., Fraser R.S.S. 1974; Early events in the germination of S. pombe ascospores.. In Cell Cycle Controls pp. 167–180 Padilla G.M., Cameron I.L., Zimmerman A.M. Edited by New York: Academic Press.;
    [Google Scholar]
  11. Padilla G.M., Carter B.L.A., Mitchison J.M. 1975; Germination of Schizosaccharomyces pombe spores separated by zonal centrifugation.. Experimental Cell Research 93:325–330
    [Google Scholar]
  12. Rousseau P., Halvorson H.O. 1973a; Physiological changes following the breaking of dormancy of Saccharomyces cerevisiae ascospores.. Canadian Journal of Microbiology 19:547–555
    [Google Scholar]
  13. Rousseau P., Halvorson H.O. 1973b; Effect of metabolic inhibitors on germination and outgrowth of Saccharomyces cerevisiae ascospores.. Canadian Journal of Microbiology 19:1311–1318
    [Google Scholar]
  14. Rousseau P., Halvorson H. O. 1973c; Macromolecular synthesis during the germination of Saccharomyces cerevisiae spores.. Journal of Bacteriology 113:1289–1295
    [Google Scholar]
  15. Steele S.D., Miller J.J. 1974; Ultrastructural changes in germinating spores of Saccharomyces cerevisiae. . Canadian Journal of Microbiology 20:923–933
    [Google Scholar]
  16. Steele S.D., Miller J.J. 1977; Amino acid uptake and protein synthesis in germinating spores of Saccharomyces cerevisiae. . Canadian Journal of Microbiology 23:407–412
    [Google Scholar]
  17. Tingle M.A., Küenzi M.J., Halvorson H.O. 1974; Germination of yeast spores lacking mitochondrial deoxyribonucleic acid.. Journal of Bacteriology 117:89–93
    [Google Scholar]
  18. Van Etten J.L. 1969; Protein synthesis during fungal spore germination.. Phytopathology 59:1060–1064
    [Google Scholar]
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