Partial Purine Deprivation Causes Sporulation of in the Presence of Excess Ammonia, Glucose and Phosphate Free

Abstract

In strains of able to synthesize purines massive sporulation is suppressed by the combination of excess ammonia, glucose and phosphate. Purine auxo-trophs, blocked in the general or the guanine-specific portion of the branched purine pathway, sporulated in such a medium when the purine required for normal growth was removed from the medium. The resulting spore titre and the sporulation frequency increased with the residual growth rate in the purine-free medium, i.e. with the leakiness of the purine mutation. Sporulation was further increased by allowing residual growth in growth-limiting amounts of guanosine. Non-leaky purine mutants blocked before 5′-phosphoribosyl-5-amino-4-imidazole carboxamide also sporulated well when supplied with 5-amino-4-imidazole carboxamide at concentrations (2 m) that supported growth at a suboptimal rate.

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1979-11-01
2024-03-29
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