Formate and Oxalate Metabolism in Free

Abstract

strain H16 when grown on formate or oxalate as the sole source of carbon and energy had doubling times between 3·5 and 4·5 h. The respective molar growth yields ( ) were 2·35 and 3·9. During growth on formate or oxalate both a soluble and a membrane-bound formate dehydrogenase were formed. The key enzymes of autotrophic CO fixation, ribulose-5-phosphate kinase and ribulosebisphosphate carboxylase, were formed during growth on formate but not on oxalate. Oxalate induced the synthesis of the enzymes of the glycerate pathway. Mutants impaired in autotrophic CO fixation but unaffected in the synthesis of the formate dehydrogenases lost their ability to grow on formate but not to grow on oxalate, giving further evidence that formate was assimilated via CO.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-115-1-185
1979-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/115/1/mic-115-1-185.html?itemId=/content/journal/micro/10.1099/00221287-115-1-185&mimeType=html&fmt=ahah

References

  1. Anthony C. 1975; The biochemistry of methylotrophic microorganisms. Science Progress 62:167–206
    [Google Scholar]
  2. Blackmore M. A., Quayle J. R., Walker I. O. 1968; Choice between autotrophy and heterotrophy in Pseudomonas oxalaticus: utilization of oxalate by cells after adaptation from growth on formate to growth on oxalate. Biochemical Journal 107:699–704
    [Google Scholar]
  3. Bowien B., Mayer F., Codd G. A., Schlegel H. G. 1976; Purification, some properties and quaternary structure of the d-ribulose 1,5-diphosphate carboxylase of Alcaligenes eutrophus . Archives of Microbiology 110:157–166
    [Google Scholar]
  4. Chandra T. S., Shethna Y. I. 1975; Oxalate and formate metabolism in Alcaligenes and Pseudomonas species. Antonie van Leeuwenhoek 41:465–477
    [Google Scholar]
  5. Chandra T. S., Shethna Y. I. 1977; Oxalate, formate, formamide, and methanol metabolism in Thiobacillus novellus . Journal of Bacteriology 131:389–398
    [Google Scholar]
  6. Cox R. B., Quayle J. R. 1975; The autotrophic growth of Micrococcus denitrificans on methanol. Biochemical Journal 150:569–571
    [Google Scholar]
  7. Dawson K. A., Allison M. J. 1977; Anaerobic oxalate degradation and methanogenesis by enrichment culture of rumen bacteria. Abstracts of the Annual Meeting of the American Society for Microbiology p. 180
    [Google Scholar]
  8. Dijkhuizen L., Wiersma M., Harder W. 1977; Energy production and growth of Pseudomonas oxalaticus OX1 on oxalate and formate. Archives of Microbiology 115:229–236
    [Google Scholar]
  9. Friedrich B., Schlegel H. G. 1975; Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. II. The isolation and characterization of mutants auxotrophic for phenylalanine and tyrosine. Archives of Microbiology 103:141–149
    [Google Scholar]
  10. Goldberg I., Rock J. S., Ben-Bassat A., Mateles R. I. 1976; Bacterial yields on methanol, methylamine, formaldehyde, and formate. Biotechnology and Bioengineering 18:1657–1668
    [Google Scholar]
  11. Gottschal J. C., Kuenen J. G. 1977; Growth of Thiobacillus A2 on mixtures of thiosulfate and formate. Proceedings of the Society for General Microbiology 474–75
    [Google Scholar]
  12. Harder W., Attwood M. M., Quayle J. R. 1973; Methanol assimilation by Hyphomicrobium sp. Journal of General Microbiology 78:155–163
    [Google Scholar]
  13. Kaltwasser H. 1968; Harnsäureabbau und Biosynthese der Enzyme Uricase, Glyoxylatcarboligase und Urease bei Hydrogenomonas H16. I. Bildung von Glyoxylatcarboligase und d-Glycerat-3-Dehydrogenase. Archiv für Mikrobiologie 64:71–84
    [Google Scholar]
  14. Namsaraev B. B., Nozhevnikova A. N., Zavarzin G. A. 1971; Utilization of formic acid by hydrogen bacteria. Mikrobiologiya English translation 40:772–776
    [Google Scholar]
  15. Quayle J. R. 1966; Glyoxylate dehydrogenase. Methods in Enzymology 9:342–346
    [Google Scholar]
  16. Quayle J. R. 1972; The metabolism of one-carbon compounds by microorganisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  17. Quayle J. R., Ferenci T. 1978; Evolutionary aspects of autotrophy. Microbiological Reviews 42:251–773
    [Google Scholar]
  18. Quayle J. R., Keech D. B. 1959; Carbon assimilation by Pseudomonas oxalaticus (OX 1). I. Formate and carbon dioxide utilization during growth on formate. Biochemical Journal 72:623–630
    [Google Scholar]
  19. Quayle J. R., Pfennig N. 1975; Utilization of methanol by Rhodospirillaceae . Archives of Microbiology 102:193–198
    [Google Scholar]
  20. Sahm H., Cox R. B., Quayle J. R. 1976; Metabolism of methanol by Rhodopseudomonas acidophila . Journal of General Microbiology 94:313–322
    [Google Scholar]
  21. Schink B., Schlegel H. G. 1978; Hydrogen metabolism in aerobic hydrogen oxidizing bacteria. Biochimie 60:297–305
    [Google Scholar]
  22. Schlegel H. G., Kaltwasser H., Gottschalk G. 1961; Ein submersverfahern zur Kultur wasserstoffoxidierender Bakterien: Wachstums-physiologische Untersuchungen. Archiv für Mikrobiologie 38:209–222
    [Google Scholar]
  23. Schneider K., Schlegel H. G. 1976; Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H16. Biochimica et biophysica acta 452:66–80
    [Google Scholar]
  24. Sineriz F., Wolin M. J. 1977; Continuous culture of methanogenic bacteria in a formate mineral medium. Abstracts of the Annual Meeting of the American Society for Microbiology p. 252
    [Google Scholar]
  25. Stokes J. E., Hoare D. S. 1969; Reductive pentose cycle and formate assimilation in Rhodopseudomonas palustris . Journal of Bacteriology 100:890–894
    [Google Scholar]
  26. Van Verseveld H. W., Stouthamer A. H. 1978; Growth yields and the efficiency of oxidative phosphorylation during autotrophic growth of Paracoccus denitrificans on methanol and formate. Archives of Microbiology 118:21–26
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-115-1-185
Loading
/content/journal/micro/10.1099/00221287-115-1-185
Loading

Data & Media loading...

Most cited Most Cited RSS feed