1887

Abstract

Binding of concanavalin A to the cell surface of developing zoospores was explored by fluorescence microscopy, electron microscopy and radioactive labelling. After labelling with fluorescein isothiocyanate-conjugated concanavalin A, the fluorescence intensity of individual non-induced zoospores varied greatly. On the other hand, similarly labelled zoospores, induced with K ions for synchronous development, revealed a more even distribution of fluorescence intensity. The zoospore surface contained 4·3 × 10 concanavalin A binding sites per cell which were randomly distributed and closely attached to the surface. The affinity constants ranged from 7.5 × 10 to 3·5 × 10 , while the Scatchard plot was typical of heterogeneous binding. Further developed cells, round cells and germlings contained 3.0 × 10 to 2·1 × 10 concanavalin A binding sites per cell which were mostly loosely associated patches protruding into the extracellular region. The affinity constants decreased appreciably compared with those measured at the zoospore stage. Specific concanavalin A binding characteristics appeared to correlate with the respective developmental stage of the zoospore.

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/content/journal/micro/10.1099/00221287-115-1-145
1979-11-01
2024-04-19
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References

  1. Gremo F., Kobiler D., Barondes S. H. 1978; Distribution of an endogenous lectin in the developing chick optic tectum. Journal of Cell Biology 19:491–499
    [Google Scholar]
  2. Gunther G. R., Wang J. L., Yahara I., Cunningham B. A., Edelman G. M. 1973; Concanavalin A derivatives with altered biological activities. Proceedings of the National Academy of Sciences of the United States of America 70:1012–1016
    [Google Scholar]
  3. Jen C. J., Haug A. 1979; Concanavalin A induced lysis of zoospores of Blastocladiella emersonii . Experimental Cell Research 120:425–428
    [Google Scholar]
  4. Lovett J. S. 1975; Growth and differentiation of the water mold Blastocladiella emersonii: cyto-differentiation and the role of ribonucleic acid and protein synthesis. Bacteriological Reviews 39:345–404
    [Google Scholar]
  5. Mills G. L., Cantino E. C. 1978; The lipid composition of the Blastocladiella emersonii γ-particle and the function of the γ-particle lipid in chitin formation. Experimental Mycology 2:99–109
    [Google Scholar]
  6. Myers R. B., Cantino E. C. 1974; The gamma particle. In Monographs in Developmental Biology 8 pp. 15–20 Wolky A. Edited by New York: S. Karger;
    [Google Scholar]
  7. Rosenthal H. E. 1967; A graphic method for the determination and presentation of binding para-meters in a complex system. Analytical Biochemistry 20:525–532
    [Google Scholar]
  8. Sharon N., Lis H. 1975; Use of lectins for the study of membranes. In Methods in Membrane Biology 3 pp. 147–200 Korn E. D. Edited by New York: Plenum Press;
    [Google Scholar]
  9. Soll D. R., Sonneborn D. R. 1971; Zoospore germination in Blastocladiella emersonii: cell differentiation without protein synthesis?. Proceedings of the National Academy of Sciences of the United States of America 68:459–463
    [Google Scholar]
  10. Soll D. R., Sonneborn D. R. 1972; Zoospore germination in Blastocladiella emersonii. IV. Ion control over cell differentiation. Journal of Cell Science 10:315–333
    [Google Scholar]
  11. Soll D. R., Bramberg R., Sonneborn D. R. 1969; Zoospore germination in the water mold, Blastocladiella emersonii. I. Measurement of germination sequence of subcellular morphological changes. Developmental Biology 20:183–217
    [Google Scholar]
  12. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  13. Truesdell L. C., Cantino E. C. 1971; The induction and early events of germination in the zoospore of Blastocladiella emersonii . Current Topics in Development Biology 6:1–44
    [Google Scholar]
  14. Weeks G. 1975; Studies of the cell surface of Dictyostelium discoideum during differentiation: the binding of [125I]-Con A to the cell surface. Journal of Biological Chemistry 250:6706–6710
    [Google Scholar]
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